ABSTRACT: Use of the Ion Torrent PGM for Determining the Genomic Sequences of Francisella and Coxiella-Like Endosymbionts and Rickettsia Directly from Hard Ticks
Project description:Use of the Ion Torrent PGM for Determining the Genomic Sequences of Francisella and Coxiella-Like Endosymbionts and Rickettsia Directly from Hard Ticks
Project description:Use of the Ion Torrent PGM for Determining the Genomic Sequences of Francisella and Coxiella-Like Endosymbionts and Rickettsia Directly from Hard Ticks
Project description:Use of the Ion Torrent PGM for Determining the Genomic Sequences of Francisella and Coxiella-Like Endosymbionts and Rickettsia Directly from Hard Ticks
Project description:Use of the Ion Torrent PGM for Determining the Genomic Sequences of Francisella and Coxiella-Like Endosymbionts and Rickettsia Directly from Hard Ticks
Project description:Use of the Ion Torrent PGM for Determining the Genomic Sequences of Francisella and Coxiella-Like Endosymbionts and Rickettsia Directly from Hard Ticks
Project description:Applications of transcriptomics have significantly advanced the identification of numerous tick salivary molecules involved in feeding and the transmission of tick-borne pathogens. In this study, we analyze the salivary gland transcriptome of Dermacentor andertsoni, the vector of Rickettsia rickettsii in the U.S., at single cell resolution.
Project description:Ticks (Acari: Ixodida) feed exclusively on blood which lacks essential nutrients such as vitamins and cofactors. These deficiencies are presumably complemented through specific symbiotic microorganisms such as Coxiella – Like Endosymbionts (CLEs) of Rhipicephalus ticks. CLE are localized in specialized host tissue cells within the Malpighian tubules (Mt) and the ovaries (Ov) from which they are maternally transmitted to developing oocytes. These two organs differ in function and cell types, and possibly also in activities performed by CLE occupants. To get insights into CLE functions within these two organs, we used comparative proteomics of CLEs in Mt and Ov of unfed R. sanguineus ticks
Project description:Ticks are vectors of different pathogens causing human and animal diseases. Particularly, Rickettsia slovaca is zoonotic infectious bacterium transmitted by Dermacentor ticks, agent of tick-borne lymphadenopathy (TIBOLA), common across Europe. Current studies point to extreme complexity of bacterial induced effects in tick host. Systems biology tools, including proteomics, greatly contribute to understanding of molecular details of pathogen-tick-host interactions. Herein we compared laboratory-infected ticks with uninfected control after four weeks of incubation. Propagation of R. slovaca was confirmed by quantitative PCR. Using DNA was confirmed infection with R. slovaca. By proteomic approach, we discovered 33 differentially abundant gel spots, 23 of them accumulated upon artificial infection with R. slovaca. Modest 6.9% of tick proteome was affected. The protein localizations showing that eight proteins spots might be secreted, three cytoplasmic, two mitochondrial, six likely having multiple localizations, one cell membrane and one nucleus. We identified following proteins defensin, serpins, glycine-rich protein, heat shock protein involved in artificially infected tick vector, Dermacentor reticulatus. Discovered differentially abundant proteins should be further evaluated as targets to block the transmission of bacterial pathogen.