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Time series total RNA sequencing of a differentiation of human embryonic stem cells towards trophoblast lineage

ABSTRACT: Time series total RNA sequencing of a differentiation of human embryonic stem cells towards trophoblast lineage

PROVIDER: PRJNA414247 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6171350.fastq.gz	Fastqsanger.gz
	SRR6171360.fastq.gz	Fastqsanger.gz
	SRR6171370.fastq.gz	Fastqsanger.gz
	SRR6171380.fastq.gz	Fastqsanger.gz
	SRR6171390.fastq.gz	Fastqsanger.gz

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Time series total RNA sequencing of a differentiation of human embryonic stem cells towards trophoblast lineage

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Project description:Differentiation of CD4+T-cells into effector subsets is a critical component of the adaptive immune system and an incorrect response can lead to autoimmunity or immune deficiency. Cellular differentiation including T-cell differentiation is accompanied by large-scale epigenetic remodeling, including changes in DNA methylation at key regulators of T-cell differentiation. The TET family of enzymes were recently shown to be able to catalyse methylated cytosine (5mC) into 5-hydroxymethylcytosine (5hmC) enabling a pathway of active removal of DNA methylation. Here, we characterize 5hmC, 5mC and transcriptional dynamics during human CD4+T-cell polarisation in a time series approach and relate these changes to profiles in ex-vivo CD4+memory subsets. We observed large-scale remodelling during early CD4+T-cell differentiation which was predictive of subsequent changes during late time points, these changes were also related to disease associated regions which we show can act as functional regulatory elements. This dataset was designed to assess how gene expression changes over time during human CD4+T-cell polarization towards Th1 and Th2. DNA methylation was assessed in relationship to 5hmC levels and changes (see data series), we observed that regions gaining 5hmC early was highly predictive of regions losing DNA methylation during late time points. This submission contains data from the DNA methylation by array profiling of human CD4+T-cells in-vitro polarized towards Th1 and Th2 time-series. It is part of series containing 5hmC and DNA methylation profiling of the same samples. See related experiments E-MTAB-4685, E-MTAB-4686, E-MTAB-4687, E-MTAB-4689.

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