Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Time series total RNA sequencing of a differentiation of human embryonic stem cells towards trophoblast lineage

ABSTRACT: Time series total RNA sequencing of a differentiation of human embryonic stem cells towards trophoblast lineage

PROVIDER: PRJNA414247 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6171350.fastq.gz	Fastqsanger.gz
	SRR6171360.fastq.gz	Fastqsanger.gz
	SRR6171370.fastq.gz	Fastqsanger.gz
	SRR6171380.fastq.gz	Fastqsanger.gz
	SRR6171390.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Time series total RNA sequencing of a differentiation of human embryonic stem cells towards trophoblast lineage

Project description:Human embryonic stem cells (hESC) can be differentiated towards progeny resembling trophoblast upon exposure to BMP4. Here we analysed transcriptional changes in cells undergoing commitment to trophoblast at 6 time points using total RNA sequencing and identified early and late regulators of trophoblast fate.

2017-10-14 | GSE104969 | GEO

Global expression profiling time series of early human embryonic stem cell differentiation towards the mesoderm lineage

Project description:Human pluripotent stem cells (hPSCs) is a promising cell type with capacities of self-renewal and pluripotency, and they can differentiate into in principal any cell types in the body. To fully make use of this unique cell type and develop efficient and reproducible differentiation protocols, more knowledge is needed about regulatory mechanisms and molecular pathways important to direct the differentiation towards specific functional cell types. The aim of this experiment was to study the events and regulators governing early differentiation human embryonic stem cells (hESCs) towards the mesoderm lineage. To this end, global expression profiling was performed for 11 time points on the hESC line SA121 (Takara-Clontech). Three biological replicates (A, B and C) were taken at day 0 (before differentiation) and on each day thereafter for ten days.

2017-06-08 | E-MTAB-5219 | biostudies-arrayexpress

Daily ocean time-series

Project description:Daily ocean time-series 2011 at San Pedro Ocean time-series

| PRJEB10834 | ENA

PICO time series metagenomes

Project description:PICO time series metagenomes

| PRJNA643505 | ENA

The Trophoblast Compartment Helps Maintain Embryonic Pluripotency and Delays Differentiation towards Cardiomyocytes

Project description:The Trophoblast Compartment Helps Maintain Embryonic Pluripotency and Delays Differentiation towards Cardiomyocytes

| PRJNA988165 | ENA

Bulk RNA-seq of a time series of hPSCs as they differentiate towards left ventricle cardiomyocytes

Project description:Bulk RNA-seq of a time series of hPSCs as they differentiate towards left ventricle cardiomyocytes

| PRJNA839709 | ENA

Time series RNA-seq of Naïve CD4 positive T-cells undergoing differentiation towards T-helper 1.

Project description:Time series RNA-seq of Naïve CD4 positive T-cells undergoing differentiation towards T-helper 1.

| PRJEB31881 | ENA

Hypoxia directs human villous CTB differentiation towards the EVT lineage in a HIF-dependent manner.

Project description:We showed that hypoxia directs first trimester primary villous cytotrophoblast (vCTB) differentiation preferentially towards HLAG+ extravillous trophoblast (EVT). Infection of primary vCTB with ARNT-specific shRNA attenuates this effect, suggesting a role of the intact HIF-complex in hypoxia-directed CTB differentiation into EVT.

2017-02-01 | GSE80995 | GEO

Eucalyptus xylem diurnal time series

Project description:Eucalyptus xylem diurnal time series

| PRJNA106035 | ENA

DNA methylation profiling of human CD4+T-cells in-vitro polarized towards Th1 and Th2 time-series

Project description:Differentiation of CD4+T-cells into effector subsets is a critical component of the adaptive immune system and an incorrect response can lead to autoimmunity or immune deficiency. Cellular differentiation including T-cell differentiation is accompanied by large-scale epigenetic remodeling, including changes in DNA methylation at key regulators of T-cell differentiation. The TET family of enzymes were recently shown to be able to catalyse methylated cytosine (5mC) into 5-hydroxymethylcytosine (5hmC) enabling a pathway of active removal of DNA methylation. Here, we characterize 5hmC, 5mC and transcriptional dynamics during human CD4+T-cell polarisation in a time series approach and relate these changes to profiles in ex-vivo CD4+memory subsets. We observed large-scale remodelling during early CD4+T-cell differentiation which was predictive of subsequent changes during late time points, these changes were also related to disease associated regions which we show can act as functional regulatory elements. This dataset was designed to assess how gene expression changes over time during human CD4+T-cell polarization towards Th1 and Th2. DNA methylation was assessed in relationship to 5hmC levels and changes (see data series), we observed that regions gaining 5hmC early was highly predictive of regions losing DNA methylation during late time points. This submission contains data from the DNA methylation by array profiling of human CD4+T-cells in-vitro polarized towards Th1 and Th2 time-series. It is part of series containing 5hmC and DNA methylation profiling of the same samples. See related experiments E-MTAB-4685, E-MTAB-4686, E-MTAB-4687, E-MTAB-4689.

2016-07-11 | E-MTAB-4688 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data