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Circulating microRNA signature as novel biomarkers for osteoarthritis development

ABSTRACT: Circulating microRNA signature as novel biomarkers for osteoarthritis development

PROVIDER: PRJNA414440 | ENA |

REPOSITORIES: ENA

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Circulating microRNA signature as novel biomarkers for osteoarthritis development

Project description:Introduction: MicroRNAs (miRNAs) in circulation have emerged as promising biomarkers. In this study we aimed to identify a circulating miRNA signature for osteoarthritis (OA) patients. Methods: Serum samples were collected from 12 primary OA patients and 12 healthy individuals and were screened using the Agilent Human miRNA Microarray. Receiver Operating Characteristic (ROC) curves were constructed to evaluate the diagnostic performance of the deregulated miRNAs. Expression levels of selected miRNAs were validated by quantitative Real-time PCR (qRT-PCR) in all serum samples and in articular cartilage samples from OA patients (n=12) and healthy individuals (n=7). Bioinformatics analysis was used to investigate the involved pathways and target genes of the above miRNAs. Results: We identified 279 differentially expressed miRNAs in the serum of OA patients compared to healthy controls. 205 (73.5%) were up-regulated and 74 (26.5%) down-regulated. ROC analysis revealed that 77 miRNAs had area under the curve (AUC)> 0.8 and p<0.05. Bioinformatics analysis in 7 out of the 77 selected miRNAs (hsa-miR-33b-3p, hsa-miR-4284, hsa-miR-671-3p, hsa-miR-663a, hsa-miR-140-3p, hsa-miR-150-5p and hsa-miR-1233-3p) revealed that their target genes were involved in multiple signaling pathways, among which FOXO, mTOR, pI3K/akt, lipid metabolism and TGF-β. A serum miRNA signature including three down-regulated miRNAs (hsa-miR-33b-3p, hsa-miR-671-3p and hsa-miR-140-3p) were also verified by qRT-PCR in OA patients. Furthermore, we found that hsa-miR-140-3p, hsa-miR-671-3p and potentially hsa-miR-33b-3p expression levels were consistently down-regulated in articular cartilage of OA patients compared to healthy individuals. Conclusions: A global miRNA serum signature was revealed in OA patients. We identified a three- miRNA signature in peripheral serum which could be potential osteoarthritis biomarkers.

2017-10-17 | GSE105027 | GEO

circulating microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis

Project description:We compared the circulating microRNA expression profiles of KBD, osteoarthritis (OA), rheumatoid arthritis (RA) and healthy controls. Blood specimens were collected from 3 KBD patients, 3 OA patients, 3 RA patients and 3 healthy controls. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array.

2016-06-01 | E-MTAB-3514 | biostudies-arrayexpress

Sequencing identifies a distinct signature of circulating microRNAs in early radiographic knee osteoarthritis

Project description:OBJECTIVE: MicroRNAs act locally and systemically to impact osteoarthritis pathophysiology, but comprehensive profiling of the circulating miRNome in early versus late stages of osteoarthritis has yet to be conducted. Our objective was to sequence the plasma miRNome from 91 patients with early [Kellgren-Lawrence grade 0 or 1 (n=41)] or late [Kellgren-Lawrence grade 3 or 4 (n=50)] symptomatic radiographic knee osteoarthritis to identify unique microRNA signatures in each disease state. MicroRNA libraries were prepared using the QIAseq miRNA Library Kit and sequenced on the Illumina NextSeq550. Counts were produced for microRNAs captured in miRBase and for novel microRNAs.

2020-10-07 | GSE151341 | GEO

Sequencing identifies a distinct signature of circulating microRNAs in early radiographic knee osteoarthritis

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Circulating microRNA profiles in early-stage osteoarthritis and rheumatoid arthritis

Project description:Osteoarthritis (OA) and rheumatoid arthritis (RA) are prevalent forms of arthritis. Early detection of OA and RA is challenging with existing methods, which can delay effective management. MicroRNAs are small molecules that have emerged as promising disease biomarkers with the potential to improve early detection and differentiation of arthritis subtypes. In this study we aimed to identify distinct circulating microRNAs in plasma from individuals with early OA and early RA, using an unbiased microRNA-sequencing approach. For microRNA-sequencing, plasma samples were collected from three study groups including: (a) early OA (N=12), individuals with knee OA symptoms and radiographic Kellgren-Lawrence grade 0 or 1; (b) early RA (N=6), treatment-naïve individuals with <6 months of RA symptoms in any joint; and (c) non-OA/RA (N=44), individuals with no history of arthritis. RNA isolated from N=62 plasma samples was sequenced using a 75-base single-end read protocol at a depth of approximately 17 ± 2.5 million reads/sample on an Illumina NextSeq2000 sequencer, followed by analysis for known and novel microRNAs using a previously optimized pipeline.

2025-08-05 | GSE283762 | GEO

Investigation of MicroRNA Biomarkers in Equine Distal Interphalangeal Joint Osteoarthritis

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Circulating microRNA profiles in early-stage osteoarthritis and rheumatoid arthritis

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Utility of circulating serum miRNAs as biomarkers of early cartilage degeneration in animal models of post-traumatic osteoarthritis and inflammatory arthritis

Project description:The purpose of this study was to identify potential serum microRNA (miRNA) biomarkers of cartilage degeneration comparing preclinical mouse models of post-traumatic osteoarthritis and inflammatory arthritis.

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Investigation of MicroRNA Biomarkers in Equine Distal Interphalangeal Joint Osteoarthritis

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