Project description:The increased fitness when grown with pyruvate as carbon source in response to lanthanum supplementation was used as starting point to study genes induced in Beijerinckiaceae bacterium RH AL1 upon exposure to lanthanum.
Project description:We investigated lanthanide (Ln) utilization in Beijerinckiaceae bacterium RH AL1, which requires light Lns (La–Nd) for methanol oxidation. The strain was grown with methanol as the carbon and energy source and with Ln sources including natural minerals (apatite, bastnäsite, gadolinite, loparite, monazite, xenotime), an alloy (ferrocerium), and synthetic compounds (La₂O₃, Nd₂O₃, LaPO₄). We did two rounds of incubations and cultures were grown to mid-exponential phase. Total RNA was extracted and mRNA enriched by means of subtractive hybridization, before samples were subjected to sequencing library preparation for downstream Illumina high-throughbut sequencing. Pre-processed and trimmed data was subsequently used for differential gene expression analysis.
Project description:Beijerinckiaceae bacterium RH AL1 was grown exponentially in with methanol (1% [v/v]) as carbon source and lanthanum (1µM) as necessary growth supplement. Harvested biomass was subjected to RNA extraction, mRNA-enrichment and Illumina sequencing library preparation for subsequent RNA-Seq analysis. The scope of this gene expression analysis was to validate the expression of genes linked to pathways that are involved in C1-metabolism.