Project description:We report the methylome sequencing and annotation of Burkholderia pseudomallei D286 based on high-throughput profiling using PacBio SMRT technology
Project description:Burkholderia pseudomallei can adapt to and thrive in a variety of environments, including soil and water, and also can infect different hosts, including humans, leading to the tropical disease melioidosis. Modulation of gene and protein expression is one of this pathogen's adaptive survival mechanisms, which could lead to changes in the bacteria's cell membrane, metabolism, and virulence. To better understand bacterial adaptation and host-pathogen interactions, this study compared the expression profiles of B. pseudomallei from infected mice to B. pseudomallei cultivated in soil extract media. B. pseudomallei in vivo was created by infecting mice through the intraperitoneal route and harvesting the spleens on day 5 post infection. Total RNA was isolated and sequenced from the harvested spleen. Sequence reads were mapped to the B. pseudomallei UKMD286 strain genome sequence.
Project description:Burkholderia pseudomallei is a Gram-negative pathogen responsible for melioidosis, a life-threatening disease throughout the region of Southeast Asia. LysR-type transcriptional regulators (LTTRs) are key regulators of bacterial virulence and metabolism, yet many remain uncharacterized. This study investigates the function of a novel LTTR, UKMD286_5923, in B. pseudomallei UKM D286. A deletion mutant was constructed, and transcriptomic analysis via RNA sequencing identified 45 upregulated and 22 downregulated genes in the mutant compared to the wild type. Functional enrichment analysis highlighted significant roles in nitrogen metabolism, host entry, and transcriptional regulation. Biofilm formation assays revealed increased biofilm production in the mutant, suggesting a regulatory role in bacterial adhesion.