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Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing (batch RNA-Seq)

ABSTRACT: Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing (batch RNA-Seq)

PROVIDER: PRJNA416037 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6224500.fastq.gz	Fastqsanger.gz
	SRR6224510.fastq.gz	Fastqsanger.gz
	SRR6224501.fastq.gz	Fastqsanger.gz
	SRR6224511.fastq.gz	Fastqsanger.gz
	SRR6224502.fastq.gz	Fastqsanger.gz

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Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing

Project description:Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing

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Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing (single cell RNA-Seq)

Project description:Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing (single cell RNA-Seq)

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Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing (batch RNA-Seq)

Project description:FACS sorted 4 bulk cell population from mouse femur (P6) were subjected to RNA sequencing. The samples were periosteal stem cell (PSC), progenitor population type 1 (PP1), progenitor population 2 (PP2) and CTSK-mGFP negative mesenchymal stem cells (MSC). The aim of this project was to obtain Differentially expressed gene list for each cell types as well as compare transcriptinal profile between each population.

2018-08-13 | GSE106235 | GEO

Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing

Project description:Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing

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Aged induced periosteal changes during early fracture healing

Project description:The periostieum is a fiberous network on the outside of the bones crucial to fracture healing. Here we present our scRNA-seq data from intact and fractured periosteal cells, collected three days after injury, from young adult and aged mice.

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YAP regulates periosteal expansion in early fracture repair.

Project description:Periosteal expansion is a key process in the early stages of bone fracture repair. The periosteum is typically quiescent, but upon fracture it expands, periosteal cells proliferate and contribute to the formation of a cartilaginous callus . The early expansion of the periosteum is tightly regulated at the transcriptional level. However, the molecular mechanisms behind periosteal expansion are unknown. Here, we show that Yes-Associated Protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) mediate periosteal expansion and periosteal cell proliferation. Bone fracture results in increased numbers of YAP-expressing periosteal cells. Deletion of YAP from Osterix (Osx) expressing periosteal cells impairs early periosteal expansion. Mechanistically, YAP regulates both cell intrinsic and extrinsic transcriptional programs that allow for periosteal expansion. Specifically, we identified Bmp4 as a cell extrinsic factor regulated by YAP, that rescues the impairment of periosteal expansion upon YAP deletion. Together, these data establish YAP mediated molecular mechanisms that allow for periosteal expansion in the early stages of fracture repair.

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Aged induced periosteal changes during early fracture healing

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Transcriptional profiling of intramembranous and endochondral ossification after fracture in mice

Project description:Bone fracture repair represents an important clinical challenge with nearly 1 million non-union fractures occurring annually in the U.S. Gene expression differs between non-union and healthy repair, suggesting there is a pattern of gene expression that is indicative of optimal repair. Despite this, the gene expression profile of fracture repair remains incompletely understood. In this work, we used RNA-seq of two well-established murine fracture models to describe gene expression of intramembranous and endochondral bone formation. We used top differentially expressed genes, enriched gene ontology terms and pathways, callus cellular phenotyping, and histology to describe and contrast these bone formation processes across time. Intramembranous repair, as modeled by ulnar stress fracture, and endochondral repair, as modeled by femur full fracture, exhibited vastly different transcriptional profiles throughout repair. Stress fracture healing had enriched differentially expressed genes associated with bone repair and osteoblasts, highlighting the strong osteogenic repair process of this model. Interestingly, the PI3K-Akt signaling pathway was one of only a few pathways uniquely enriched in stress fracture repair. Full fracture repair involved a higher level of inflammatory and immune cell related genes than did stress fracture repair. Full fracture repair also differed from stress fracture in a robust downregulation of ion channel genes following injury, the role of which in fracture repair is unclear. This study offers a broad description of gene expression in intramembranous and endochondral ossification across several time points throughout repair and suggests several potentially intriguing genes, pathways, and cells whose role in fracture repair requires further study

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Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing

Project description:This SuperSeries is composed of the SubSeries listed below.

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Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing

Project description:Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing

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