Allium mongolicum
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ABSTRACT:
PROVIDER: PRJNA416814 | ENA |
REPOSITORIES: ENA
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Project description:Seeds, as an effective depository of plant genetic resources and an important raw material for germplasm innovation, aging or deterioration will directly lead to a reduction in germination rate, vigor and viability, inhibit the normal development of the seed embryo and seedling growth. Methods: Allium mongolicum seeds stored naturally for 2, 4 and 5 years were used as test materials, determination of germination indexes, antioxidant enzyme activities and other physiological indexes in seeds treated with 0.8 mmol/L spermidine solution, and proteomic and transcriptomic sequencing were performed. Results: The results showed that the germination ability of Allium mongolicum seeds decreased continuously with the extension of storage life, and the germination rate was only about 40% after 5 years of storage. The relative conductivity, MDA and H2O2 content were significantly higher, CAT, POD, SOD, APX, and respiratory rate were significantly reduced; Spd treatment increased seed germination rate, significantly reduced MDA, H2O2 content and relative conductivity, and increased CAT, POD, SOD, and APX activities. Functional analysis of the differentially expressed proteins showed that the differentially expressed proteins were mainly concentrated in energy metabolism pathways such as carbon metabolism, amino acid biosynthesis, glycolysis/glycogen isomerization, TCA cycle, pyruvate metabolism and pentose phosphate metabolism. Transcriptome sequencing yielded similar results. PPI analysis identified 10 proteins related to the aging of Allium mongolicum seeds, and the core protein was also verified by qPCR, the gene expression trend was basically consistent with the proteomic assay results. Conclusions : Energy metabolism may be a key pathway in the aging of Allium mongolicum seeds, and spermidine can effectively alleviate Allium mongolicum seed aging. The results enriched the molecular mechanism of seed storability of Allium mongolicum and provided theoretical basis for molecular marker-assisted breeding of storability traits in Allium mongolicum L.
2024-11-18 | PXD058020 |
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