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Transcriptomic responses of Arabidopsis wild-type and amp1 seedlings after hyperphyllin treatment

ABSTRACT: Transcriptomic responses of Arabidopsis wild-type and amp1 seedlings after hyperphyllin treatment

PROVIDER: PRJNA417711 | ENA |

REPOSITORIES: ENA

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Transcriptomic responses of Arabidopsis wild-type and amp1 seedlings after hyperphyllin treatment [AraGene-1_1-st array]

Project description:ALTERED MERISTEM PROGRAM1 (AMP1) is a member of the M28 family of carboxypeptidases with a pivotal role in plant development and stress adaptation. Its most prominent mutant defect is a unique hypertrophic shoot phenotype combining a strongly increased organ formation rate with enhanced meristem size and the formation of ectopic meristem poles. However, so far the role of AMP1 in shoot development could not be assigned to a specific molecular pathway nor is its biochemical function resolved. We used a chemical genetic approach to identify the drug hyperphyllin (HP), which specifically mimics the shoot defects of amp1, including plastochron reduction and enlargement and multiplication of the shoot meristem. To further assess whether hyperphyllin acts in an AMP1-dependent manner we compared the transcriptonal responses of hyperphyllin-treated wild-type and amp1 mutant seedlings.

2017-11-08 | GSE106659 | GEO

Transcriptomic responses of Arabidopsis wild-type and amp1 seedlings after hyperphyllin treatment [AraGene-1_1-st array]

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Transcriptomic analysis of Hyperphyllin-treated Arabidopsis seedlings [ATH1 array]

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Arabidopsis ila3 mutant seedlings versus wild-type seedlings.

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Transcriptomic analysis of amp1-13 and cyp78a5,7 Arabidopsis seedlings

Project description:ALTERED MERISTEM PROGRAM1 (AMP1) is a member of the M28 family of carboxypeptidases with a pivotal role in cell fate maintenance in the embryo and shoot meristem. A defect in AMP1 function results in suspensor to embryo conversion and a hypertrophic shoot meristem forming ectopic stem cell pools. However, so far the role of AMP1 in shoot development could not be assigned to a specific molecular pathway nor is its biochemical function resolved. Double mutants in CYP78A5 and CYP78A7 develop a similar set of cell fate defects. To further assess whether this phenotypic overlap is also depicted in a congruency at the global gene expression level, we analyzed the transcriptomic responses of both genotypes

2020-09-15 | GSE147160 | GEO

Transcriptom analyses of Arabidopsis altered meritem program1-32 (amp1-32) mutant as well as wild-type Columbia-0 plants via next-generation sequencing (NGS).

Project description:Purpose:The goals of this study are to compare transcriptome of amp1-32 mutant with that of wild-type Columbia-0 plant and to identify genes whose expression are tightly regualted by AMP1 in Arabidopsis. Methods: mRNA profiles of 3-week-old wild-type Col-0 and amp1-32 mutant plants were generated by deep sequencing, in duplicate, using Illumina HiSeq2000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with a method : TopHat followed by Cufflinks. Results: Using an optimized data analysis workflow, we mapped about 30~53 million sequence reads per sample to the Arabidopsis genome (TAIR10). From the 2 biological replicates, we detected 135 up-regulated genes, and 36 down-regulated genes, in the amp1-32 mutant plant. Conclusions: Our study represents the first detailed analysis of transcriptome of amp1 mutant plants, with biologic replicates. Our results show that AMP1 protein had weak, but significant, effects on transcription of genes important for plant development and responses to stresses.

2017-05-30 | GSE81618 | GEO

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Project description:Transcriptomic analysis of amp1-13 and cyp78a5,7 Arabidopsis seedlings

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Arabidopsis thaliana seedlings: Wild type vs. zrf1 mutants

Project description:Transcriptional profiling of 6-day-old seedlings of Arabidopsis wild type control and zrf1 mutants is performed using Agilent's Whole Arabidopsis Gene Expression Microarray (4x44K).

2016-12-31 | GSE76244 | GEO

RNA-seq analysis of wild type Columbia seedlings

Project description:RNA-seq analysis of wild type Columbia seedlings

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