Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Adult functions for the Drosophila DHR78 nuclear receptor

ABSTRACT: Adult functions for the Drosophila DHR78 nuclear receptor

PROVIDER: PRJNA418283 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6288270.fastq.gz	Fastqsanger.gz
	SRR6288271.fastq.gz	Fastqsanger.gz
	SRR6288272.fastq.gz	Fastqsanger.gz
	SRR6288273.fastq.gz	Fastqsanger.gz
	SRR6288274.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Adult functions for the Drosophila DHR78 nuclear receptor

Project description:Here we study the adult functions of the single Drosophila member of this subfamily, DHR78, with the goal of defining its ancestral functions in the absence of genetic redundancy. We show that DHR78 mutants have a shortened lifespan and reduced motility. Mated DHR78 mutant females display reduced triglycerides along with a reduced feeding rate. Transcriptional profiling reveals a major role for DHR78 in promoting the expression of genes that are abundantly expressed in the midgut, suggesting that it contributes to nutrient uptake. We also identify roles for DHR78 in maintaining the expression of genes in the ecdysone and Notch signaling pathways.

2018-02-13 | GSE106866 | GEO

Transcription profiling of Drosophila DHR96 nuclear receptor mutants

Project description:DHR96 is a Drosophila member of the CAR/PXR/LXR family of Nuclear receptors. To Identify potential target genes for the DHR96 nuclear receptor we used microarray analysis to identify genes that were mis-regulated relative to control animals in the DHR96 mutant. Experiment Overall Design: Total RNA was isolated from mature adult DHR96 mutant and genetically matched Canton S controls and subjected to affymetrix microarray analysis.

2009-10-27 | E-GEOD-18576 | biostudies-arrayexpress

Adult functions for the Drosophila DHR78 nuclear receptor.

Project description:BackgroundThe Testicular Receptors 2 and 4 (TR2, TR4) comprise a small subfamily of orphan nuclear receptors. Genetic studies in mouse models have identified roles for TR4 in developmental progression, fertility, brain development, and metabolism, as well as genetic redundancy with TR2. Here we study the adult functions of the single Drosophila member of this subfamily, DHR78, with the goal of defining its ancestral functions in the absence of genetic redundancy.ResultsWe show that DHR78 mutants have a shortened lifespan, reduced motility, and mated DHR78 mutant females display a reduced feeding rate. Transcriptional profiling reveals a major role for DHR78 in promoting the expression of genes that are expressed in the midgut, suggesting that it contributes to nutrient uptake. We also identify roles for DHR78 in maintaining the expression of genes in the ecdysone and Notch signaling pathways.ConclusionsThis study provides a new context for linking the molecular activity of the TR orphan nuclear receptors with their complex roles in adult physiology and lifespan. Developmental Dynamics 247:315-322, 2018. © 2017 Wiley Periodicals, Inc.

| S-EPMC5771960 | biostudies-literature

Effect of depletion of Drosophila Hepatocyte Nuclear Factor 4 (dHNF4) in enterocytes on gene expression in the adult midgut

Project description:The main objective of the sudy was to characterize the functions of Drosophila Hepatocyte Nuclear Factor 4 (dHNF4) in midgut enterocytes. For this, we expressed a UAS-dHNF4 RNAi transgene under the control of mex-GAL4, which is active specifically in midgut enterocytes. We then collected adult male midguts, extracted total RNA and performed mRNA-seq. Our data demonstrate that dHNF4 acts as a master regulator of intestinal lipid metabolism in Drosophila.

2024-09-05 | GSE271746 | GEO

Transcription profiling of Drosophila Hr39 mutant females reveals the sf1-related nuclear hormone receptor hr39 regulates Drosophila female reproductive tract development and function

Project description:The vertebrate nuclear hormone receptor steroidogenic factor 1 (SF1; NR5A1) controls reproductive development and regulates the transcription of steroid-modifying cytochrome P450 genes. We find that the SF1-related Drosophila nuclear hormone receptor HR39 is also essential for sexual development. In Hr39 mutant females, the sperm-storing spermathecae and glandular parovaria are absent or defective, causing sterility. Our results indicate that spermathecae and parovaria secrete reproductive tract proteins required for sperm maturation and function, like the mammalian epididymis and female reproductive tract. Hr39 controls the expression of specific cytochrome P450 genes and is required in females both to activate spermathecal secretion and repress male-specific courtship genes such as takeout. Thus, a pathway that, in vertebrates, controls sex-specific steroid hormone production, also mediates reproductive functions in an invertebrate. Our findings suggest that Drosophila can be used to model more aspects of mammalian reproductive biology than previously believed. Experiment Overall Design: Wild type and Hr39(04443) Spermathecae, Wild type and Hr39(04443) Reproductive Tract

2008-06-11 | E-GEOD-10264 | biostudies-arrayexpress

Project description:The vertebrate nuclear hormone receptor steroidogenic factor 1 (SF1; NR5A1) controls reproductive development and regulates the transcription of steroid-modifying cytochrome P450 genes. We find that the SF1-related Drosophila nuclear hormone receptor HR39 is also essential for sexual development. In Hr39 mutant females, the sperm-storing spermathecae and glandular parovaria are absent or defective, causing sterility. Our results indicate that spermathecae and parovaria secrete reproductive tract proteins required for sperm maturation and function, like the mammalian epididymis and female reproductive tract. Hr39 controls the expression of specific cytochrome P450 genes and is required in females both to activate spermathecal secretion and repress male-specific courtship genes such as takeout. Thus, a pathway that, in vertebrates, controls sex-specific steroid hormone production, also mediates reproductive functions in an invertebrate. Our findings suggest that Drosophila can be used to model more aspects of mammalian reproductive biology than previously believed. Keywords: mutant/wild-type comparison and tissue comparison

2008-04-26 | GSE10264 | GEO

Drosophila E78A nuclear receptor regulates intestinal dietary triacylglyceride uptake and systemic lipid levels

Project description:Here we show that the Drosophila nuclear receptor E78A regulates transcription in adult animals facilitating dietary lipid uptake. E78A mutant adults are viable with normal developmental timing, locomotor activity, female fecundity, but have significantly reduced stored triglycerides. This reduction in whole body triglyceride stores appears to originate from the midgut, using neutral lipid stainings we determined lipid levels in the midgut were greatly reduced. Upon examining our RNA-seq analysis we found that a gastric lipase, CG17192, is transcriptionally coordinated by E78A. CG17192 is expressed in an adult, intestinal specific fashion within control flies, and has a human ortholog. Through dietary supplementation and genetic testing we determined that the whole body hypolipidemia seen within E78A mutants is dependent on CG17192 expression within the intestine. Restoration of this lipase in the intestine of mutant flies is sufficient to restore normal lipid levels. Our data support the model that E78A contributes to a transcriptional regulation of the gastric lipase, CG17192, thus directing dietary triglyceride uptake in the adult fly.

2021-01-01 | GSE152031 | GEO

NRDE2 negatively regulates nuclear exosome functions

Project description:NRDE2 negatively regulates nuclear exosome functions

| PRJNA491937 | ENA

Transcriptional remodeling effects of the nuclear receptor NR4A2 in adult rat ventricular myocytes

Project description:Transcriptional remodeling effects of the nuclear receptor NR4A2 in adult rat ventricular myocytes

| PRJNA506897 | ENA

T cell receptor-triggered nuclear actin network formation drives CD4 T cell effector functions

Project description:T cell receptor-triggered nuclear actin network formation drives CD4 T cell effector functions

| PRJNA505494 | ENA