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Transcriptome analysis of sciatic nerve in mutant krox20 mice at 14 day post natal compared to wild-type mice

ABSTRACT: Transcriptome analysis of sciatic nerve in mutant krox20 mice at 14 day post natal compared to wild-type mice

PROVIDER: PRJNA419984 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6325560_1.fastq.gz	Fastqsanger.gz
	SRR6325560_2.fastq.gz	Fastqsanger.gz
	SRR6325561_1.fastq.gz	Fastqsanger.gz
	SRR6325561_2.fastq.gz	Fastqsanger.gz
	SRR6325562_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of sciatic nerve in mutant krox20 mice at 14 day post natal compared to wild-type mice

Project description:RNAseq was performed on sciatic nerves from controls and Krox20 mutants to elucidate the role of Krox20 inthe myelinisation process.

2019-09-03 | GSE107401 | GEO

Sciatic nerve FAIRE-chip and ChIP-Chip with Egr2/Krox20 and Sox10

Project description:Egr2/Krox20 and Sox10 regulate genes involved in formation of myelination in the peripheral nervous system. ChIP-chip assays were performed on rat sciatic nerve at P15, a peak timepoint of myelination. In addition, Faire was used to identify areas of open chromatin. This experiment includes a custom ChIP-chip design incorporating many genes that are dynamically regulated during peripheral nerve myelination. Two antibodies were used for Egr2, Abcam and Covance PRB-236P. Egr2 and Sox10 ChIP samples were hybridized along with total input. In addition, FAIRE samples were hybridized relative to input DNA

2010-10-14 | E-GEOD-23648 | biostudies-arrayexpress

Sciatic nerve FAIRE-chip and ChIP-Chip with Egr2/Krox20 and Sox10

2010-10-15 | GSE23648 | GEO

Gene expression from aged and young sciatic nerve after nerve injury

Project description:Sciatic nerve ligation was performed on cohorts of 2-month and 24-month old animals. Resulting gene-expression data were generated from sciatic nerve 1 and 4 days after injury compared to naïve animals. Results show differences in sciatic nerve responses with normal aging. Total RNA taken from sciatic nerves from 2-month and 24-month old animals at either day 0, 1 and 4 after sciatic nerve crush injury.

2014-07-30 | E-GEOD-59040 | biostudies-arrayexpress

Timecourse expression data from sciatic nerve distal stump of C57BL/6 and C57BL/6 OlaHsd-Wlds mice.

Project description:Wallerian degeneration (WD) involves the fragmentation of axonal segments disconnected from their cell bodies, segmentation of the myelin sheath, and removal of debris by Schwann cells and immune cells. The removal and downregulation of myelin-associated inhibitors of axonal regeneration and synthesis of growth factors by these two cell types are critical responses to successful nerve repair. Here, we analyzed the transcriptome of the sciatic nerve of mice carrying the Wallerian degeneration slow (WldS) mutant gene, a gene that confers axonal protection in the distal stump after injury, therefore causing significant delays in WD, neuroinflammation, and axonal regeneration. 56 C57BL6 mice and 56 C57BL/6 OlaHsd-Wlds mice were anesthetized with isoflurane and underwent a microcrush lesion of their left sciatic nerve at the mid-thigh level (exept naive mice, t0). At 0, 3, 7 and 14 days post-injury, mice were anesthetized and killed by cervical dislocation. Sciatic nerves were collected and conective tissue removed. A 4-mm long sciatic nerve segment was taken from the nerve distal stump, starting at 1 mm distal from the lesion up to 5 mm distal. Distal nerve stumps were pooled by group and RNA extracted. Samples were hybridized to GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). Biological replicate was done.

2011-06-01 | E-GEOD-22291 | biostudies-arrayexpress

Sciatic nerve crush regulation of RNA alternative poly-adenylation

Project description:Poly-adenylation regulates post-transcriptional processing and localization of mRNA. To study the effect of nerve injury on poly-adenylation across the transcriptome, 3'-end RNAseq was carried on DRG neurons following sciatic nerve injury. Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition.

2025-05-16 | GSE230721 | GEO

Gene expression from aged and young sciatic nerve after nerve injury

2014-07-30 | GSE59040 | GEO

Proximal sciatic nerve stumps exhibit sexually dimorphic transcriptome changes in mice after peripheral nerve injury (axotomy)

Project description:Transcriptional changes are distinct in female and male mice in the time course of peripheral nerve injury response. Here, we present sexually dimorphic transcriptome profiles established in the space of 24 h period after sciatic nerve axotomy.

2022-07-18 | GSE182713 | GEO

Sciatic nerve crush upregulation of B2-SINE expression at specific genomic loci

Project description:To map the loci of B2-SINE expressed in DRG which are regulated by sciatic nerve injury, paired-end RNAseq was carried on DRG neurons following sciatic nerve injury at 24h and 72h (3d) after injury. Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition.

2025-05-16 | GSE230720 | GEO

Clathrin IP_mouse sciatic nerve axons_Roylab_UCSD

Project description:Sciatic nerve axon segments from adult mouse were isolated. Following enzymatic digestion, lysate was subjected to incubation with beads conjugated with either clathrin heavy chain antibody or control IgG antibody for clathrin immunoprecipitation. MudPIT analysis was subsequently performed to identify proteins co-precipitating with clathrin.

2021-08-12 | PXD027857 | Pride

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