Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

OX40 regulates double-negative regulatory T cells dependently of its effects on conversion and function

ABSTRACT: OX40 regulates double-negative regulatory T cells dependently of its effects on conversion and function

PROVIDER: PRJNA421906 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6365685.fastq.gz	Fastqsanger.gz
	SRR6365686.fastq.gz	Fastqsanger.gz
	SRR6365687.fastq.gz	Fastqsanger.gz
	SRR6365688.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

OX40 regulates double-negative regulatory T cells dependently of its effects on conversion and function

Project description:Methods: To better understand the role of OX40 in the regulation of double-negative regulatory T cells (DNT), OX40 deficiency and wild-type DNT converted from naïve CD4 T cells were compared in a transcriptome sequencing study. Total RNA was isolated from FACS-separated DNT. Transcriptome sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and sequenced on an Illumina Hiseq platform (Illumina, San Diego, CA). Sequences were aligned to the reference genome with TopHat and processed with Cufflinks, which quantifies each transcript in each sample using reference annotations produced by the University of California Santa Cruz UCSC. Differentially expressed genes with a fold change of >=2.0 and padj < 0.05 between OX40 deficiency and control DNT were submitted to GO and KEGG enrichment analysis, which uses unbiased methods to assess pathway enrichment. Results: Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that OX40 knockout are involved in regulatory function of DNT; including suppressive function, apoptosis, proliferation, and so on. Compared to control DNT, OX40 knockout DNT exhibited up-regulation of 52 genes and down-regulated of 46 genes. The most enriched GO term were Signal Transduction, Protein Binding, and Banding.

2020-12-08 | GSE107908 | GEO

The Transcription Factor T-bet Plays a Crucial Role in Regulating the Immune Regulatory Function of Double-Negative T Cells

Project description:This study found that, compared to conventional CD4+ / CD8+ T cells, double-negative T (DNT) cells express high levels of IFN-γ, and IFN-γ can dose-dependently enhance the in vitro conversion efficiency of DNT cells, suggesting that DNT cells tend to adopt a Th1 phenotype. Single-cell sequencing revealed that DNT cells exhibit high expression of the transcription factor T-bet. Knockout of T-bet significantly downregulated IFN-γ expression in DNT cells and reduced their suppressive capacity on macrophages in vitro. Transcriptome sequencing further elucidated the specific mechanism by which T-bet regulates the immune regulatory function of DNT cells.

2026-01-01 | GSE286125 | GEO

Molecular subtyping of “double negative” MIBC

Project description:Molecular subtyping of “double negative” MIBC

| PRJNA816119 | ENA

Gene expression analysis of OX40-triggered mouse Treg

Project description:Regulatory T (Treg) maintain the tumor microenvironment in an immunosuppressive state preventing effective anti-tumor immune response. A possible strategy to overcome Treg cell suppression focuses on OX40, a costimulatory molecule expressed constitutively by Treg cells while induced in activated effector T (Teff) cells. OX40 stimulation by the agonist mAb OX86 inhibits Treg cell suppression and boosts Teff cell activation. Here we uncover the mechanisms underlying the therapeutic activity of OX86 treatment dissecting its distinct effects on Treg and on effector memory T (Tem) cells, which are the most abundant CD4+ populations strongly expressing OX40 at the tumor site. In response to OX86, tumor-infiltrating Treg cells produced significantly less interleukin 10 (IL-10), possibly in relation to a decrease in the transcription factor IRF1. Tem cells responded to OX86 by upregulating surface CD40L expression, providing a licensing signal to dendritic cells (DCs). The CD40L/CD40 axis was required for Tem cell-mediated in vitro DC maturation and in vivo DC migration. Accordingly, OX86 treatment was no longer therapeutic in CD40 KO mice. In conclusion, following OX40 stimulation, blockade of Treg cell suppression and enhancement of the Tem cell adjuvant effect both concurred to free DCs from immunosuppression and to activate the immune response against the tumor. Total RNA obtained from sorted mouse FoxP3-GFP+ Treg activated in vitro with anti-CD3 in the presence of an OX40-agonist mAb (OX86) or isotype control.

2011-09-25 | E-GEOD-32373 | biostudies-arrayexpress

Ezh2 deficiency in double negative thymocytes

Project description:Expression profiles from Rag1 mutant and Rag1/Ezh2 double mutant double negative thymocytes Keywords = Rag1 Keywords = Ezh2 Keywords = Thymocyte Keywords: repeat sample

2005-09-01 | GSE1565 | GEO

Lupus milieu favors the conversion of self-reactive CD8 T cells to IL-17 producing double negative T cells

Project description:Lupus milieu favors the conversion of self-reactive CD8 T cells to IL-17 producing double negative T cells

| PRJNA627722 | ENA

Lupus milieu favors the conversion of self-reactive CD8 T cells to IL-17 producing double negative T cells

Project description:Mature double negative (DN) T cells are αβ T cells lacking CD4/CD8 coreceptors and expanded in patients with systemic lupus erythematosus (SLE). It is not known whether they display a narrow or expander TCR repertoire.

2020-04-24 | GSE149239 | GEO

Integrated analysis of mRNA and miRNA expression profiling in double negative T cells converted from mouse CD4 T cells

Project description:To reveal the differential mechanism and the role of microRNA (miRNA) regulation in double negative T cell (DNT cell) conversion, we have analyzed the message RNA (mRNA) and miRNA expression profiles using high-throughput sequencing. Our results demonstrated that differentially expressed mRNAs and miRNAs represent extensively changes between naïve CD4 T cells, activated CD4 T cells and DNT cells.

2026-01-04 | GSE143622 | GEO

RNA-sequencing of mouse double-negative developing thymocytes

Project description:RNA-sequencing of mouse double-negative developing thymocytes

| PRJNA484209 | ENA

Gene expression analysis of Ox40-cre/ROSA-YFP CD4+ T cell subsets

Project description:An Ox40-cre allele was used for lineage marking of CD4 T cells. Naive T cells that had previously expressed OX40 demonstrated a partially activated phenotype that was distinct from that of the majority of naive T cells. These results are consistent with a minor subpopulation of naive T cells being dependent on strong signaling responses to thymic self ligands. Keywords: T cell population comparison. Characterization of a novel subpopulation of naive T cells. Four types of T cells were sorted from mice (naive, CD44lo, CD44hi and Treg).

2010-04-03 | E-GEOD-13502 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data