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Transcriptome analysis of females and males following knockdown serine/threonine protein phosphatase 1 catalytic subunit encoding genes(Sj-pp1c) in Schistosoma japonicum

ABSTRACT: Transcriptome analysis of females and males following knockdown serine/threonine protein phosphatase 1 catalytic subunit encoding genes(Sj-pp1c) in Schistosoma japonicum

PROVIDER: PRJNA428455 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6439810_1.fastq.gz	Fastqsanger.gz
	SRR6439810_2.fastq.gz	Fastqsanger.gz
	SRR6439811_1.fastq.gz	Fastqsanger.gz
	SRR6439811_2.fastq.gz	Fastqsanger.gz
	SRR6439812_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of females and males following knockdown serine/threonine protein phosphatase 1 catalytic subunit encoding genes(Sj-pp1c) in Schistosoma japonicum

Project description:Purpose: To obtain the differentially expressed genes based on post-RNAi transcriptional profiling analysis of females and males, which provide a more comprehensive overview about the effects of Sj-pp1c knockdown on schistosoma developmental and reproduction biology at the molecular level. Methods: Schistosoma japonicum females and males were collected from 21 days post infection worm pairs that untreated (Blank,BLA_FE/M) or treated with combined Sj-pp1c dsRNAs (Sj-pp1c KD,PP1_FE/M) cultured for 7 d in vitro. Total mRNA profiles of females and males were generated by deep sequencing, in triplicate, using Illumina HiSeqTM2000. Results: 41-51 million reads (average 44 million) were obtained for each sample after quality filtering, and 63-67% reads were mapped to S. japonicum reference genome(WormBase ParaSite, http://parasite.wormbase.org/ftp.html; version 7.0) and identified 15,314 transcripts by Tophat (version v2.0.12) and Cufflinks (Version 2.1.1.). 2291 and 229 transcripts showed differentially expressed in males and females, respectively,between the untreated and Sj-pp1c dsRNAs treated group, with adjust P value <0.05. GO analysis showed differentially expressed genes are mainly function in extracellular matrix structural constituent, proteolysis and collagen trimer. Conclusions: We performed analysis on transcriptional profiling of females and males following Sj-pp1c-RNAi by RNA-seq, which provided molecular evidence for the pleiotropic roles that Sj-pp1c plays in schistosome biology.

2018-06-24 | GSE108738 | GEO

Serine/Threonine phosphatase PP2A is essential for optimal B cell function

Project description:Protein phosphatase 2A (PP2A), a serine/threonine phosphatase, has been shown to control T cell function. We found that in vitro activated B cells and B cells from various lupus-prone mice and patients with systemic lupus erythematosus display increased PP2A activity. To understand the contribution of PP2A to B cell function, we generated a Cd19CrePpp2r1aflox/flox (flox/flox) mouse which lacks functional PP2A only in B cells. Flox/flox mice displayed reduced spontaneous germinal center formation and decreased responses to T-dependent and T-independent antigens while their B cells responded poorly in vitro to stimulation with an anti-CD40 antibody or CpG in the presence of IL-4. Transcriptome and metabolome studies revealed altered NAD and purine/pyrimidine metabolism and increased expression of purine nucleoside phosphorylase in PP2A-deficient B cells. Our results demonstrate that PP2A is required for optimal B cell function and may contribute to increased B cell activity in systemic autoimmunity.

2020-02-04 | GSE144700 | GEO

Serine/Threonine phosphatase PP2A is essential for optimal B cell function

Project description:Serine/Threonine phosphatase PP2A is essential for optimal B cell function

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The Serine/Threonine Protein Phosphatase 6 Is Required for Efficient Gametogenesis in Sexual-Stage Plasmodium berghei

Project description:The Serine/Threonine Protein Phosphatase 6 Is Required for Efficient Gametogenesis in Sexual-Stage Plasmodium berghei

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Cellular serine/threonine phosphatase activity during human cytomegalovirus infection.

Project description:While the importance of cellular and viral kinases in HCMV replication has been demonstrated, relatively little is known about the activity of cellular phosphatases. We conducted a series of experiments designed to investigate the effect of HCMV infection on cellular serine/threonine phosphatase activity. We found that the abundance of two major cellular serine/threonine phosphatases, PP1 and PP2A, increases during HCMV infection. This was associated with an increase in threonine phosphatase activity in HCMV-infected cells. HCMV infection conferred resistance to the effects of the phosphatase inhibitors calyculin A (CA) and okadaic acid with regards to global protein hyperphosphorylation and the shutoff of protein synthesis. The protective effect of HCMV infection could be overcome at a high concentration of CA, suggesting that cellular phosphatase activity is required for critical cellular processes during HCMV infection. Specifically, phosphatase activity was required to limit the accumulation of phospho-eIF2alpha, but not phospho-PKR, during HCMV infection.

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Transcription profiling of mouse NIH3T3 cells over-expressing RNA-dependent serine/threonine protein kinase (PKR)PKR

Project description:Global analysis of gene expression in NIH3T3 cells over-expressing RNA-dependent serine/threonine protein kinase (PKR).

2008-06-15 | E-GEOD-682 | biostudies-arrayexpress

The Serine/Threonine Protein Phosphatase 6 Is Required for Efficient Gametogenesis in Sexual-Stage Plasmodium berghei

Project description:Protein phosphorylation plays a critical role during the development of malaria parasites. Here, we performed a functional analysis of the Plasmodium berghei Ser/Thr protein phosphatase 6 (PbPP6), which is associated with the plasma membrane of macrogametes and ookinetes. Compared to wild-type P. berghei, the genetic disruption of pbpp6 (∆pbpp6) resulted in reduced asexual growth of the parasites and prolonged survival of infected mice. The ∆pbpp6 parasites showed impaired gametogenesis, particularly affecting male gametogenesis, which substantially decreased both ookinete formation and mosquito transmission. Transcriptomic analysis revealed an over 11-fold downregulation of nek3, a regulator of MAPK2 within the PKG-Ca²⁺ signaling cascade, foreshadowing pathway dysregulation that was further evidenced by significantly diminished intracellular cGMP levels, decreased cytosolic Ca²⁺ mobilization, and reduced DNA replication in activated Δpbpp6 gametocytes. Phosphoproteomic analysis detected increased phosphorylation at the Ser508 site of guanylyl cyclase alpha (GCα), indicating that PbPP6 regulates cGMP-PKG-Ca2+ signaling through modulation of GCα activity during gametogenesis. Additionally, we observed altered expression of messenger ribonucleoproteins in the Δpbpp6 parasites, which may affect the translational repression of stored mRNAs in female gametocytes and impact post-fertilization development in mosquitoes. Collectively, this study highlights the potential of targeting PP6 to disrupt malaria transmission.

2025-06-02 | GSE271105 | GEO

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