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Expansion of adult human pancreatic tissue yields organoids harbouring progenitor cells with endocrine differentiation potential

ABSTRACT: Expansion of adult human pancreatic tissue yields organoids harbouring progenitor cells with endocrine differentiation potential

PROVIDER: PRJNA428952 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6449570_1.fastq.gz	Fastqsanger.gz
	SRR6449570_2.fastq.gz	Fastqsanger.gz
	SRR6449580_1.fastq.gz	Fastqsanger.gz
	SRR6449580_2.fastq.gz	Fastqsanger.gz
	SRR6449571_1.fastq.gz	Fastqsanger.gz

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Expansion of adult human pancreatic tissue yields organoids harbouring progenitor cells with endocrine differentiation potential

Project description:Gene expression profiles from ALDH high cells sorted from expanded adult human pancreatic organoids are more similar to fetal pancreatic tissue and ALDH high cells sorted from expanded fetal human pancreatic organoids than to adult human islets or adult islet-depleted exocrine tissue.

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Expansion of Adult Human Pancreatic Tissue Yields Organoids Harboring Progenitor Cells with Endocrine Differentiation Potential.

Project description:Generating an unlimited source of human insulin-producing cells is a prerequisite to advance β cell replacement therapy for diabetes. Here, we describe a 3D culture system that supports the expansion of adult human pancreatic tissue and the generation of a cell subpopulation with progenitor characteristics. These cells display high aldehyde dehydrogenase activity (ALDHhi), express pancreatic progenitors markers (PDX1, PTF1A, CPA1, and MYC), and can form new organoids in contrast to ALDHlo cells. Interestingly, gene expression profiling revealed that ALDHhi cells are closer to human fetal pancreatic tissue compared with adult pancreatic tissue. Endocrine lineage markers were detected upon in vitro differentiation. Engrafted organoids differentiated toward insulin-positive (INS+) cells, and circulating human C-peptide was detected upon glucose challenge 1 month after transplantation. Engrafted ALDHhi cells formed INS+ cells. We conclude that adult human pancreatic tissue has potential for expansion into 3D structures harboring progenitor cells with endocrine differentiation potential.

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RNA-seq of sorted pancreatic endocrine progenitor cells

Project description:Pancreatic endocrine cells arise from a NGN3+ population during pancreas organogenesis. To gain a more thorough understanding of this progenitor pool, we used a reporter mouse - NGN3-EGFP - and sorted EGFP+ cells from e15.5 pancreata of control animals. The data generated from this experiment will allow us to visualize gene expression levels in endocrine progenitors during normal development and can be used to compare against mutant animal gene expression.

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