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Evaluation of gene expression changes in tcs08 single and double mutants in the pneumococcal strain TIGR4

ABSTRACT: Evaluation of gene expression changes in tcs08 single and double mutants in the pneumococcal strain TIGR4

PROVIDER: PRJNA428996 | ENA |

REPOSITORIES: ENA

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Evaluation of gene expression changes in tcs08 single and double mutants in the pneumococcal strain TIGR4

Project description:In order to elucidate the regulatory role of pneumococcal TCS08, a transcriptomic approach was performed using microarray from bacterial cultures in chemical defined medium of single and double tcs08 mutants. A total of 159 genes presenting significant expression changes were selected for further evaluation and discussion. These 159 genes were classified accordingly to their biochemical function.

2018-05-01 | GSE108874 | GEO

Evaluation of gene expression changes in tcs09 single and double mutants in the pneumococcal strain D39

Project description:In order to elucidate the regulatory role of pneumococcal TCS09, a transcriptomic approach was performed using RNA-seq from bacterial cultures in chemical defined medium of single and double tcs09 mutants. A total of 69 genes presenting significant expression changes were selected for further evaluation and discussion. These 69 genes were classified accordingly to their biochemical function.

2021-02-22 | GSE165642 | GEO

RNA-seq analysis of transport deficient S. pneumoniae TIGR4 mutants

Project description:The polyamine transport operon in Streptococcus pneumoniae TIGR4 is necessary for survival in murine models of pneumococcal pneumonia. To date, there is no description of polyamine transport dependent pneumococcal gene expression. In this study, we compared gene expression between the wild-type and transport deficient (potABCD) TIGR4 by RNA-Seq analysis.

2017-08-14 | E-MTAB-5951 | biostudies-arrayexpress

Evaluation of gene expression changes in tcs09 single and double mutants in the pneumococcal strain D39

Project description:Evaluation of gene expression changes in tcs09 single and double mutants in the pneumococcal strain D39

| PRJNA695258 | ENA

Impact of impaired polyamine biosynthesis on Streptococcus pneumoniae TIGR4 gene expression

Project description:The polyamine biosynthesis gene, speE, in Streptococcus pneumoniae TIGR4 is necessary for survival in murine models of pneumococcal pneumonia. To date, there is no description of polyamine biosynthesis dependent pneumococcal gene expression. In this study, we compared gene expression between the wild-type and biosynthesis deficient (speE) TIGR4 by RNA-Seq analysis.

2023-01-01 | E-MTAB-6070 | biostudies-arrayexpress

Host response to S. pneumoniae TIGR4 and ΔpotABCD

Project description:The aim of this experiment is to determine the difference in protein expression, if any, in a mouse model of pneumococcal pneumonia using wild type and a polyamine transport operon deletion mutant strain of S. pneumoniae TIGR4, using expression proteomics. Eight week old female C57bl/6 mice were used in this study. The experimental design had two groups of 3 animals each that were administered 50 µL 1 X 107 CFU wild type S. pneumoniae TIGR4 or S. pneumoniae TIGR4 ΔpotABCD strain by intranasal instillation. The control group with three animals received 50 µL PBS. Mice were euthanized 4 hr and 12 hr post infection and lung tissues were harvested. The control group animals were euthanized 12 hr post infection.

2018-10-27 | PXD002300 | Pride

Role of PsaR of Streptococcus pneumoniae D39 and TIGR4 in global gene expression and virulence

Project description:Previous studies have indicated that PsaR of Streptococcus pneumoniae is a manganese-dependent regulator, negatively affecting the expression of at least seven genes. Here, we extended these observations by transcriptome and proteome analysis of psaR mutants in strains D39 and TIGR4. The microarray analysis identified three shared PsaR targets: the psa-operon, pcpA, and prtA. Additionally, we found 31 genes to be regulated by PsaR in D39 only, most strikingly a cellobiose-specific PTS and a putative bacteriocin operon (sp0141-sp0146). In TIGR4, 14 PsaR gene targets were detected, with the rlrA pathogenicity islet being the most pronounced. Proteomics confirmed most of the shared gene targets. To examine the contribution of PsaR to pneumococcal virulence, we compared D39 and TIGR4 wild-type (wt) and psaR-mutants in three murine infection models. During colonization, no clear effect was observed of the psaR mutation in either D39 or TIGR4. In the pneumonia model, small but significant differences were observed in the lungs of mice infected with either D39wt or ∆psaR: D39∆psaR had an initial advantage in survival in the lungs. Conversely, TIGR4∆psaR-infected mice had significantly lower bacterial loads at 24h only. Finally, during experimental bacteremia, D39∆psaR-infected mice had significantly lower bacterial loads in the blood stream than wt-infected mice for the first 24h of infection. TIGR4∆psaR showed attenuation at 36h only. In conclusion, our results show that PsaR of D39 and TIGR4 has a strain-specific role in global gene expression and in the development of bacteremia in mice.

2008-11-19 | GSE13505 | GEO

Investigation of pneumococcal two-component signal transduction system RR/HK06 in TIGR4

Project description:We wanted to identify genes regulated by the two-component signal transduction system RR/HK06 in Streptococcus pneumoniae. We used microarray in order to discover regulated genes, investigating the effects of the over-expression of RR06 in TIGR4 (serotype 4). Keywords: Gene Regulation

2006-12-17 | GSE5330 | GEO

RNA-seq analysis of transport deficient S. pneumoniae TIGR4 mutants

Project description:RNA-seq analysis of transport deficient S. pneumoniae TIGR4 mutants

| PRJEB22066 | ENA

Polyamine synthesis effects capsule expression in Streptococcus pneumoniae TIGR4

Project description:Purpose: We recently reported that isogenic deletion of lysine decarboxylase (ΔcadA/SP_0916), an enzyme that catalyzes the biosynthesis of polyamine cadaverine in Streptococcus pneumoniae TIGR4 results in loss of capsular polysaccharide (CPS), which constitutes a novel mechanism of regulation of CPS. Here, we conducted RNA-Seq to elucidate molecular mechanisms of CPS regulation in polyamine synthesis impaired pneumococci. Result: Significantly differentially expressed genes in ΔcadA represent pneumococcal pathways involved in the biosynthesis of precursors for CPS and peptidoglycan. Conclusion: We establish a possible link and interchange between two cellular processes such as high energy demanding capsule production and oxidative stress responses in polyamine synthesis impaired pneumococci (ΔcadA).

2019-09-30 | GSE130511 | GEO

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