Project description:Profiled the transcriptional response to over-expression Manganese-SOD (MnSOD) in adult Drosophila. A doxycycline-regulated system was employed to induce MnSOD over-expression, resulting in mean and maximal lifespan increases of ~ 20%. Examination of the genome-wide response to this lifespan intervention provided key insights into the molecular basis of aging. Keywords: genetic modification; MnSOD over-expression
Project description:Genome-wide association studies (GWAS) and fine-mapping have identified several distinct variants within HNF1B associated with risk of prostate cancer, but its mechanism of action in this context is unknown. To determine the effects of HNF1B in prostate cancer, we over-expressed HNF1B in PC3 cells to identify biological pathways affected by this change, and performed in vitro assays to validate our findings.