Project description:Metagenomic sequencing of mice with different treatments: Mice were randomly divided into donor control group (Donor + MRS), constipation model group (STC + MRS), or a Lactobacillus acidophilus treated group (STC + La): A humanized mouse model was established by intragastric administration of fecal bacterial liquid from healthy donors or STC patients on alternate days, followed by continuous administration of Lactobacillus acidophilus in treatment group. Finally, the feces of each group of mice were collected, and the intestinal microbial communities of the mice were analyzed through metagenomic sequencing. 16S rRNA sequencing of mice before and after the use antibiotics: Before and after treating the mice with antibiotics, the mice's feces were collected for 16s rRNA sequencing respectively.
Project description:In the present study, a quantitative proteomic approach was used to analyse and compare the proteome in horns from endangered species (rhinoceros, Saiga antelope, and Tibetan antelope) and common species (yak, water buffalo, and goat) based on the isobaric tag for relative and absolute quantification (iTRAQ) techniques. In total, 591 proteins were identified, and 321 were quantified and categorised based on molecular function, cellular component, and biological process. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) results based on differences in the amount of protein identified three major clusters, and proteins including transglutaminase, desmocollin, and elongation factors were selected as trait components from proteomic patterns of horn samples from different species. Quantitative proteomic analysis-based strategies can therefore provide further evidence for sustainable alternatives to replace animal horn from threatened species.
2022-03-02 | PXD010901 | Pride
Project description:Metagenomic analysis of microbial communities in domestic yak, Tibetan antelope and Tibetan wild ass in 2023
| PRJNA1421066 | ENA
Project description:Metagenomic analysis of microbial communities in domestic yak, Tibetan antelope and Tibetan wild ass in 2021
| PRJNA1132386 | ENA
Project description:ITS gene sequencing of Domestic yak, Tibetan antelope, and Tibetan wild ass
| PRJNA1131646 | ENA
Project description:16s rRNA gene sequencing of Domestic yak, Tibetan antelope, and Tibetan wild ass
| PRJNA1131634 | ENA
Project description:metagenome data of sable antelope feces
