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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

ABSTRACT: Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

PROVIDER: PRJNA432598 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6664520.fastq.gz	Fastqsanger.gz
	SRR6664530_1.fastq.gz	Fastqsanger.gz
	SRR6664530_2.fastq.gz	Fastqsanger.gz
	SRR6664521.fastq.gz	Fastqsanger.gz
	SRR6664531_1.fastq.gz	Fastqsanger.gz

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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [ChIP-seq]

Project description:Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [ChIP-seq]

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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [RNA-seq]

Project description:Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [RNA-seq]

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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [RNA-seq]

Project description:Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3–4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.

2018-02-11 | GSE110036 | GEO

Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [ChIP-seq]

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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts.

Project description:Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3-4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.

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Derivation of formative-like pluripotent stem cells from mammalian embryos [RNA-Seq]

Project description:Two phases of pluripotency, naïve and primed, have been captured in vitro and studied in details1. A third formative phase was recently proposed to exist between naïve and primed phases2. Formative pluripotency entails permissiveness for direct primordial germ cell (PGC) induction and competence for blastocyst chimeras, and is characterized by transcriptional and epigenetic features intermediate of naïve and primed pluripotency. To date, however, stable pluripotent stem cells (PSCs) harboring formative features haven’t been derived from early mammalian embryos. Here we develop a method which enabled the derivation and culture of stable formative-like embryonic stem cells (ESCs) from mouse blastocysts. Formative-like mouse ESCs share molecular features characteristic of early post-implantation epiblasts and are competent for PGC-like cell induction and blastocyst chimera formation. The same culture also supported the derivation of ESCs and transgene-free induced pluripotent stem cells (iPSCs) from horse blastocysts and fibroblasts, respectively. Horse ESCs/iPSCs transcriptionally resembled mouse formative cells, and could also be directly induced into PGC-like cells. Formative-like horse iPSCs could efficiently chimerize horse, mouse, goat, sheep and pig embryos. Stable formative-like PSCs will be invaluable for studying mammalian pluripotency, and our method may be broadly applicable for the derivation of PGC and chimera competent PSCs from other mammalian species.

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Derivation of formative-like pluripotent stem cells from mammalian embryos [ChIP-Seq]

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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-02-11 | GSE110040 | GEO

Derivation of embryonic stem cells from cloned blastocysts using improved somatic cell nuclear transfer in common marmoset

Project description:The common marmoset (Callithrix jacchus) is a genetically modifiable non-human primate increasingly used in biomedical research. However, methods to preserve or expand valuable genetic resources in this species remain underdeveloped. Here, we established a method for deriving embryonic stem cells (ESCs) from blastocysts generated by somatic cell nuclear transfer (SCNT) in the marmoset. Injection of mRNA encoding the histone demethylase Kdm4d enabled efficient reprogramming of somatic nuclei, allowing blastocyst formation in 14.5% from fibroblasts. However, nuclear transfer ESCs (ntESCs) were not established from these blastocysts. Combining this method with a G9a histone methyltransferase inhibitor (RK-701) improved blastocyst quality and allowed derivation of ntESC lines, including wildtype and GFP-transgenic lines. These ntESCs exhibited normal karyotypes and pluripotency both in vitro and in vivo. Nuclear and mitochondrial DNA analyses confirmed their nuclear donor origin and cytoplasmic inheritance from recipient oocytes. Transcriptome analysis identified abnormally expressed genes in ntESCs present in a line-dependent and -independent manner, suggesting partial reprogramming resistance that may inform future optimization of SCNT in primates. Our study establishes marmoset SCNT method enabling derivation of ntESCs which may provide a new platform for preserving and engineering marmoset genetic resources.

2025-09-29 | GSE305965 | GEO

Derivation of Porcine Extraembryonic Endoderm-like Cells from from Blastocysts

Project description:Derivation of Porcine Extraembryonic Endoderm-like Cells from from Blastocysts

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