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The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis

ABSTRACT: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis

PROVIDER: PRJNA432954 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6672400.fastq.gz	Fastqsanger.gz
	SRR6672393.fastq.gz	Fastqsanger.gz
	SRR6672394.fastq.gz	Fastqsanger.gz
	SRR6672395.fastq.gz	Fastqsanger.gz
	SRR6672396.fastq.gz	Fastqsanger.gz

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The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis

Project description:knockdown of HEN1 in Nematostella results in a developmental arrest. Small RNA sequencing revealed that the levels of both miRNAs and piRNAs drop dramatically in the morphant animals.

2019-01-01 | GSE110148 | GEO

The methyltransferase HEN1 is required for microRNA and piRNA stability as well as larval metamorphosis in Nematostella vectensis

Project description:The methyltransferase HEN1 is required for microRNA and piRNA stability as well as larval metamorphosis in Nematostella vectensis

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The methyltransferase HEN1 is required for microRNA and piRNA stability as well as larval metamorphosis in Nematostella vectensis

Project description:Knockdown of Dicer and PIWI2 in Nematostella results in a developmental arrest. Small RNA sequencing revealed that the levels of both miRNAs and piRNAs drop dramatically in the morphant animals.

2019-01-01 | GSE115184 | GEO

The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis.

Project description:Small non-coding RNAs (sRNAs) such as microRNAs (miRNAs), small interfering RNAs (siRNAs) and piwi-interacting RNAs (piRNAs) regulate the levels of endogenous, viral and transposable element RNA in plants (excluding piRNAs) and animals. These pathways are explored mainly in bilaterian animals, such as vertebrates, arthropods and nematodes, where siRNAs and piRNAs, but not miRNAs bind their targets with a perfect match and mediate the cleavage of the target RNA. Methylation of the 3' ends of piRNAs and siRNAs by the methyltransferase HEN1 protects these sRNAs from degradation. There is a noticeable selection in bilaterian animals against miRNA-mRNA perfect matching, as it leads to the degradation of miRNAs. Cnidarians (sea anemones, corals, hydroids and jellyfish), are separated from bilaterians by more than 600 million years. As opposed to bilaterians, cnidarian miRNAs frequently bind their targets with a nearly perfect match. Knowing that an ortholog of HEN1 is widely expressed in the sea anemone Nematostella vectensis, we tested in this work whether it mediates the stabilization of its sRNAs. We show that the knockdown of HEN1 in Nematostella results in a developmental arrest. Small RNA sequencing revealed that the levels of both miRNAs and piRNAs drop dramatically in the morphant animals. Moreover, knockdown experiments of Nematostella Dicer1 and PIWI2, homologs of major bilaterian biogenesis components of miRNAs and piRNAs, respectively, resulted in developmental arrest similar to HEN1 morphants. Our findings suggest that HEN1 mediated methylation of sRNAs reflects the ancestral state, where miRNAs were also methylated. Thus, we provide the first evidence of a methylation mechanism that stabilizes miRNAs in animals, and highlight the importance of post-transcriptional regulation in non-bilaterian animals.

| S-EPMC6114907 | biostudies-literature

MAPK signaling promotes embryonic neural induction and expression of embryonic patterning genes in Nematostella vectensis

Project description:While FGF mediated MEK/ERK signaling is required for apical tuft formation and metamorphosis in the sea anemone Nematostella vectensis (Rentzsch et al, 2008), nothing is known about the role of MEK/ERK signaling in inducing germ layers and cell types during early developmental stages. We therefore performed a genome wide expression array on UO126 (MEK inhibitor) treated blastula stages compared to DMSO treated control embryos and identified genes potentially involved in neurogenesis, germ layer specification and axial patterning.We performed transcriptional profiling of Nematostella vectensis blastula stages (24 hours post fertilisation @ 17C) using a custom made whole genome array (4x72K - A-MEXP-2380). DMSO treated wild-type embryos were compared to U0126 (MEK Inhibitor) treated embryos at the blastula stage.

2016-06-07 | E-MTAB-4831 | biostudies-arrayexpress

Nematostella vectensis

Project description:Nematostella vectensis Genome sequencing

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Nematostella vectensis

Project description:Nematostella vectensis RAD sequencing

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Nematostella vectensis

Project description:Nematostella vectensis Raw sequence reads

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Project description:Nematostella vectensis Raw sequence reads