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Identification of the tumour transition states occurring during EMT

ABSTRACT: Identification of the tumour transition states occurring during EMT

PROVIDER: PRJNA433523 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6691230.fastq.gz	Fastqsanger.gz
	SRR6691240.fastq.gz	Fastqsanger.gz
	SRR6691250.fastq.gz	Fastqsanger.gz
	SRR6691260.fastq.gz	Fastqsanger.gz
	SRR6691270.fastq.gz	Fastqsanger.gz

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Identification of the tumour transition states occurring during EMT

Project description:Identification of the tumour transition states occurring during EMT

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Identification of the tumour transition states occurring during EMT [ATAC-seq]

Project description:EMT (epithelial-mesenchymal transition) in cancer has been associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However,direct in vivo evidence supporting this possibility is still lacking. By screening a large panel of cell surface markers, we identified the existence of multiple tumour subpopulations associated with different EMT stages from epithelial to completely mesenchymal states passing through intermediate hybrid states in skin and mammary primary tumours. Although all EMT subpopulations presented similar tumour propagating cell capacity, they displayed different, invasiveness and metastatic potential. Their transcriptional and epigenetic landscapes defined by RNA-seq and ATAC-seq identified the underlying gene regulatory networks, transcription factors and signalling pathways that control these different EMT transition states. Finally, these tumour subpopulations are localized in different niches that differentially regulateEMT transition states.

2018-02-15 | GSE110584 | GEO

Identification of the tumour transition states occurring during EMT [ATAC-seq]

Project description:Identification of the tumour transition states occurring during EMT [ATAC-seq]

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Identification of the tumour transition states occurring during EMT [RNA-seq]

Project description:Identification of the tumour transition states occurring during EMT [RNA-seq]

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Identification of the tumour transition states occurring during EMT [RNA-seq]

Project description:EMT in cancer has been associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However,direct in vivo evidence supporting this possibility is still lacking. By screening a large panel of cell surface markers, we identified the existence of multiple tumour subpopulations associated with different EMT stages from epithelial to completely mesenchymal states passing through intermediate hybrid states in skin and mammary primary tumours. Although all EMT subpopulations presented similar tumour propagating cell capacity, they displayed different , invasiveness and metastatic potential. Their transcriptional and epigenetic landscapes defined by RNA-seq and ATAC-seq identified the underlying gene regulatory networks, transcription factors and signalling pathways that control these different EMT transition states. Finally, these tumour subpopulations are localized in different niches that differentially regulateEMT transition states.

2018-02-15 | GSE110585 | GEO

Identification of the tumour transition states occurring during EMT [p63 overexpression RNA-seq]

2018-02-15 | GSE110586 | GEO

Identification of the tumour transition states occurring during EMT [p63 overexpression RNA-seq]

Project description:Identification of the tumour transition states occurring during EMT [p63 overexpression RNA-seq]

| PRJNA434065 | ENA

Identification of the tumour transition states occurring during EMT

Project description:scRNAseq of YFP+ Epcam+ and Epcam- skin squamous cell carcinoma cells

2018-04-19 | GSE110357 | GEO

Identification of the tumour transition states occurring during EMT

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-02-15 | GSE110587 | GEO

Jag1-regulated hybrid-EMT state identifies a subset of CD9hi pancreatic tumour-initiating cells with enriched stemness

Project description:We previously identified a pancreatic ductal adenocarcinoma (PDAC) subpopulation capable of initiating PDAC and giving rise to PDAC heterogeneity, marked by the tetraspanin CD9. PDAC shows great cellular heterogeneity, with pronounced epithelial and mesenchymal cancer cell populations, and it has been suggested that cancer cells with hybrid epithelial-mesenchymal transition (EMT) features peculiarly contribute to tumour aggressiveness. Here, we identify a stable hybrid-EMT subpopulation that is highly enriched for tumour initiating cell (TIC) properties which constituted a subpopulation of CD9hi TICs1. Hybrid-EMT CD9hi TICs showed increased capacity to form organoids and generate epithelial and mesenchymal tumour cell progeny. Interestingly, in contrast to the majority of PDAC organoids with cystic morphology, hybrid-EMT organoids exhibited a solid morphology and were able to give rise to both cystic and solid organoids, implying a relationship between stemness and solid morphology. Cell depletion of hybrid-EMT cells led to gradual collapse of organoids formation capability, suggesting hybrid-EMT cells are required for long-term organoid formation. Mechanistically, hybrid-EMT cells upregulated Jag1, which promoted their self-renewal by activating the Notch signalling pathway. Thus, the hybrid EMT state is a defining feature of stem cells in PDAC.

2025-10-01 | GSE245863 | GEO

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