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Expression Data from CD4-CD25+regulatory T cells of VAVcreKeapfl/fl mice

ABSTRACT: Expression Data from CD4-CD25+regulatory T cells of VAVcreKeapfl/fl mice

PROVIDER: PRJNA434722 | ENA |

REPOSITORIES: ENA

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Expression Data from CD4-CD25+regulatory T cells of VAVcreKeapfl/fl mice

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Expression Data from CD4+CD25+Nrp1+ regulatory T cells of Foxp3creCREBfl/fl mice

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Expression Data from CD4+CD25+Nrp1+ regulatory T cells of Foxp3creCREBfl/fl mice

Project description:Regulatory T cells (Tregs) are gatekeepers of immune homeostasis and characterized by expression of Foxp3, which maintains Treg identity. The transcriptional activator CREB critically stabilizes Foxp3 expression in vitro. Here we demonstrate that in mice with a Foxp3-specific knockout of CREB, Tregs show a reduced Foxp3 expression in vivo, but surprisingly enhanced expression of IL-13, IL-10, ST-2 and CREM. This rendered such Tregs highly suppressive in vitro and prevents disease activity in Th1 models like T cell mediated transfer colitis in an IL-10 dependent way. On the other hand, type 2 B cell responses were enhanced resulting in high IgE levels and susceptibility towards experimental asthma. Our data suggest that CREB expression in Tregs is important for the balance between Th1 and Th2 responses by regulating ST-2 and IL-10. We used microarrays to detail the global programme of gene expression in CD4+CD25+Nrp1+ T cells of Foxp3creCREBfl/fl mice

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Gene expression profiling of CD4+CD25+Foxp3+ regulatory T cells and CD4+CD25-Foxp3- T cells in the course of Plasmodium yoelii infection of BALB/c mice

Project description:The objective of the present study was to characterize the phenotype of CD4+CD25+Foxp3+ regulatory T cells (Tregs) in the course of parasitic Plasmodium yoelii (P .yoelii) infection of BALB/c mice. Therefore we performed microarray expression analysis of CD4+CD25+Foxp3+ Tregs isolated by FACS from spleens of non-infected mice and from spleens of mice infected with P. yoelii 3 days and 5 days post infection. By comparing the gene expression profiles, we were able to identify molecules which were differentially expressed by Tregs during parasitic infection and thereby might be involved in their immune-suppressive function. Moreover, we included CD4+CD25-Foxp3- T cells from spleens of non-infected and P. yoelii-infected mice in our analysis. It was proposed that immune-suppressive CD4+CD25-Foxp3- T cells might be induced during Plasmodium infection of mice. Thus, detailed gene expression data of these cells in comparison to CD4+CD25+Foxp3+ Tregs would contribute a better understanding in the phenotype. FACS sorted CD4+CD25+Foxp3+ Tregs and CD4+CD25-Foxp3- T cells from pooled spleens of non-infected Foxp3/ eGFP mice (served as reference) and from pooled spleens of P. yoelii infected Foxp3/ eGFP mice 3 days and 5 days post infection were analyzed as single probes.

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CD4/CD25 subsets of mouse spleen

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RNA-seq from mice CD4+CD25+ splenocytes

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mRNA expression in CD4+ CD25+ GITR+ regulatory T cells from WT and Bach2 KO mice

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