Project description:Paper title: Awakening ancient polar Actinobacteria: diversity, evolution and specialized metabolite potential Author List: Natalie Millan-Aguinaga, Sylvia Soldatou, Sarah Brozio, John T. Munnoch, John Howe, Paul A. Hoskisson, Katherine R. Duncan Citation: Microbiology, 2019,165(11) DOI:10.1099/mic.0.000845

2020-01-07 | MSV000084763 | MassIVE

Ammonia oxidizing archaeal community composition in Gulf of Mexico sediments investigated with an amoA functional gene microarray

Project description:Ammonia-oxidizing archaeal (AOA) amoA diversity and relative abundance in Gulf of Mexico sediments (0-2 cm) were investigated using a functional gene microarray; a two color array with a universal internal standard Two color array (cy3 and cy5): the universal standard 20 bp oligo (fluoresced with cy5) is printed to the slide with a 70-mer. Environmental DNA sequences (fluoresced with Cy3) within 15% of the 70-mer will bind to it. Signal is the cy3/cy5. Up to four arrays per sample, with two biological replicates made into two targets, each run on duplicate arrays.

2013-03-01 | E-GEOD-42286 | biostudies-arrayexpress

Ammonia oxidizing archaeal community composition in Gulf of Mexico sediments investigated with an amoA functional gene microarray

2013-03-01 | GSE42286 | GEO

marine sediment metagenome

Project description:marine sediments Targeted loci environmental

| PRJNA305190 | ENA

sediment metagenome

Project description:Sewer sediments Targeted loci environmental

| PRJNA297575 | ENA

Light enhances growth in non-phototropic Actinobacteria (Rhodoluna lacicola strain Ta8)

Project description:Light is a source of energy and an environmental cue that is available in excess in most surface environments. In prokaryotic systems, conversion of light to energy by photoautotrophs and photoheterotrophs is well understood, but the conversion of light to information and the cellular response to that information has been characterized in only a few species. Our goal was to explore the response of freshwater Actinobacteria, which are ubiquitous in illuminated aquatic environments, to light. We found that Actinobacteria without functional photosystems grow faster in the light, likely because sugar transport and metabolism are upregulated in the light, while protein synthesis is upregulated in the dark. Based on the action spectrum of the growth effect, and comparisons of the genomes of three Actinobacteria with this growth rate phenotype, we propose that the photosensor in these strains is a putative CryB-type cryptochrome. The ability to sense light and upregulate carbohydrate transport during the day could allow these cells to coordinate their time of maximum organic carbon uptake with the time of maximum organic carbon release by primary producers.

2018-07-07 | GSE116706 | GEO

Light enhances growth in non-phototropic Actinobacteria (Aurantimicrobium sp. MWH-Mo1)

2018-07-07 | GSE116705 | GEO