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Microprocessor depends on hemin to recognize the apical loop of primary microRNA

ABSTRACT: Microprocessor depends on hemin to recognize the apical loop of primary microRNA

PROVIDER: PRJNA436986 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6932950.fastq.gz	Fastqsanger.gz
	SRR6932941.fastq.gz	Fastqsanger.gz
	SRR6932951.fastq.gz	Fastqsanger.gz
	SRR6932942.fastq.gz	Fastqsanger.gz
	SRR6932952.fastq.gz	Fastqsanger.gz

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Microprocessor depends on hemin to recognize the apical loop of primary microRNA

Project description:Microprocessor, which consists of a ribonuclease III DROSHA and its cofactor DGCR8, initiates microRNA (miRNA) maturation by cleaving primary miRNA transcripts (pri-miRNAs). We recently demonstrated that the DGCR8 dimer recognizes the apical elements of pri-miRNAs, including the UGU motif, to accurately locate and orient Microprocessor on pri-miRNAs. However, the mechanism underlying the selective RNA binding remains unknown. In this study, we find that hemin, a ferric ion-containing porphyrin, enhances the specific interaction between the apical UGU motif and the DGCR8 dimer, allowing Microprocessor to achieve high efficiency and fidelity of pri-miRNA processing in vitro. Furthermore, by generating a DGCR8 mutant cell line and carrying out rescue experiments, we discover that hemin preferentially stimulates the expression of miRNAs possessing the UGU motif, thereby conferring differential regulation of miRNA maturation. Our findings reveal the molecular action mechanism of hemin in pri-miRNA processing and establish a novel function of hemin in inducing specific RNA-protein interaction.

2018-03-26 | GSE111431 | GEO

Microprocessor depends on hemin to recognize the apical loop of primary microRNA.

| S-EPMC6009577 | biostudies-literature

Hemin and iron transcriptome of Porphyromonas gingivalis (part 1)

Project description:Transcriptional profiling of P. gingivalis cells comparing cells grown under hemin replete conditions to that grown under hemin depleted conditions Two-condition experiment, hemin replete vs. hemin deplete grown cells. Biological replicates: 3 independently grown and harvested bacterial cells. Four technical replicates (four replicates per array).

2015-05-18 | E-GEOD-50701 | biostudies-arrayexpress

Microprocessor recruitment to elongating RNA Polymerase II is required for differential expression of microRNAs

Project description:The cellular abundance of mature microRNAs (miRNAs) is dictated by the efficiency of nuclear processing of primary miRNA transcripts (pri-miRNAs) into pre-miRNA intermediates. The Microprocessor complex of Drosha and DGCR8 carries this out, but it has been unclear what controls Microprocessor's differential processing of various pri-miRNAs. Here, we show that Drosophila DGCR8 (Pasha) directly associates with the C terminal domain of the RNA polymerase II elongation complex when it is phosphorylated by the Cdk9 kinase (pTEFb). When association is blocked by loss of Cdk9 activity, a global change in pri-miRNA processing is detected. Processing of pri-miRNAs with a UGU sequence motif in their apical junction domain increases, while processing of pri-miRNAs lacking this motif decreases. Therefore, phosphorylation of RNA polymerase II recruits Microprocessor for co-transcriptional processing of non-UGU pri-miRNAs that would otherwise be poorly processed. In contrast, UGU-positive pri-miRNAs are robustly processed by Microprocessor independent of RNA polymerase association.

2017-09-15 | GSE103234 | GEO

Hemin and iron transcriptome of Porphyromonas gingivalis (part 1)

Project description:Transcriptional profiling of P. gingivalis cells comparing cells grown under hemin replete conditions to that grown under hemin depleted conditions

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Effect of interleukin-10 on primary oligodendrocyte progenitor cells under hemin administration

Project description:To investigate the gene expression changes in primary oligodendrocyte progenitor cells generated from wild-type mouse pups after hemin or hemin+interleukin-10 or hemin+interleukin-10+stattic treatment

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An intrinsically disordered region of Drosha selectively promotes miRNA biogenesis, independent of tissue-specific Microprocessor condensates

Project description:Precise control of miRNA biogenesis is of extreme importance, since mis-regulation of miRNAs underlies or exacerbates many disease states. The Microprocessor complex, composed of DROSHA and DGCR8, carries out the first of two cleavage steps in canonical miRNA biogenesis. Despite recent advances in understanding the molecular mechanism of Microprocessor, the role of N-terminal regions of DROSHA is less characterized due their high intrinsic disorder. Here we demonstrate that Microprocessor forms liquid-liquid phase separated (LLPS) condensates in select tissues in C. elegans. We find that an intrinsically disordered region (IDR) near the N-terminus of DRSH-1/DROSHA is required for normal development and biogenesis of a subset of miRNAs. Despite the known role of IDRs in LLPS, the role of the DRSH-1 IDR in miRNA biogenesis and development is genetically separable from its contribution to granule formation. A cis region of an IDR-dependent miRNA confers IDR-dependence to another miRNA, suggesting that the IDR may recognize sequences or structures in the miRNA transcript. Future studies will further elucidate the specificity of this interaction and the putative role of Microprocessor condensates.

2025-07-20 | GSE289120 | GEO

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