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MiRNA expression profiles of chicken embryonic visceral tissue infected with F48E9 or La Sota

ABSTRACT: miRNA expression profiles of chicken embryonic visceral tissue infected with F48E9 or La Sota

PROVIDER: PRJNA438448 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6839621.fastq.gz	Fastqsanger.gz
	SRR6839622.fastq.gz	Fastqsanger.gz
	SRR6839623.fastq.gz	Fastqsanger.gz

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miRNA expression profiles of chicken embryonic visceral tissue infected with F48E9 or La Sota

Project description:Three cDNA libraries of mixed visceral tissues from F48E9, La Sota, or uninfected chicken embryos were constructed, and small-RNA deep sequencing was conducted to detect the expression levels of small-RNAs. Intergroup comparisons were used to identify changes in miRNA expression caused by NDV infection. La Sota affected the expression of 61 miRNAs (36 upregulated and 25 downregulated) at 36 hpi, and F48E9 infection altered the expression levels of 66 miRNAs (33 upregulated and 31 downregulated).

2018-06-06 | GSE111868 | GEO

Chicken embryonic visceral tissue with F48E9 or La Sota

Project description:Chicken embryonic visceral tissue with F48E9 or La Sota

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RNA sequencing-based analysis of the visceral tissue transcriptome of chicken embryo infected with F48E9 or La Sota

Project description:We attempted to characterize the transcriptome of the chicken embryo during Newcastle disease virus (NDV) infection using RNA-sequencing analysis. The cDNAs derived from Total RNA of the pooled visceral tissues infected with F48E9 or La Sota were sequenced and analysed. The collected clean reads covered about 4.02% (2,341,868 reads) of the entire F48E8 reference sequence, while only 0.02% reads (13,886) were mapped to the La Sota genome. RNA-Seq datasets from groups La Sota, F48E9 and control, were respectively mapped to 71.76%, 68.55% and 70.05% of the reference genome Galgal 4.73. Compared with the control, 2,035 and 1,604 differentially expressed genes of hosts were found responding to F48E9 and La Sota infection, respectively. GO and KEGG pathway enriched various signalling pathways with elements playing roles in enhancing or preventing viral infection, like IFP35, NMI, Mx, OAS*A, IFITM5, STAT1 and IFNβ. So far, we know that velogenic NDV made far more transcripts during infection and caused significant impact on the host, showing a large number of genes in various pathways at high levels of expression.

2018-06-06 | GSE111867 | GEO

RNA sequencing-based analysis of the visceral tissue transcriptome of chicken embryo infected with F48E9 or La Sota

Project description:RNA sequencing-based analysis of the visceral tissue transcriptome of chicken embryo infected with F48E9 or La Sota

| PRJNA438447 | ENA

Chicken embryonic visceral tissue with F48E9 or La Sota

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-06-06 | GSE111869 | GEO

Common microRNA⁻mRNA Interactions in Different Newcastle Disease Virus-Infected Chicken Embryonic Visceral Tissues.

Project description:To investigate the roles and explore the altered expression of microRNAs (miRNAs) and mRNAs in chicken embryos in response to Newcastle disease virus (NDV) infection, deep sequencing was performed. Then, a conjoint analysis of small RNA-seq and mRNA-seq was performed to screen interactional miRNA⁻mRNA pairs during NDV infection. In total, 15 and 17 up- and downregulated miRNAs were identified that potentially targeted 4279 and 6080 mRNAs in NDV-infected chicken embryonic tissues, respectively; in addition, 595 upregulated and 480 downregulated mRNAs were identified. The conjoint analysis of the obtained data identified 1069 miRNA⁻mRNA pairs. Among these pairs, 130 pairs were related to immune or inflammatory responses. The relationship between gga-miR-203a and its target transglutaminase 2 (TGM2) was confirmed using a dual-luciferase reporter system and a real time quantitative polymerase chain reaction (RT-qPCR) assay. Overall, the discovery of miRNAs, mRNAs, and their potential pairing relationships, which may be involved in the regulation of NDV infection, will facilitate our understanding of the complex regulatory relationship between the host and the virus.

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Project description:Drosophila melanogaster La, La autoantigen-like, is expressed in 2 baseline experiment(s);

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