Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantitative reverse transcription PCR analysis of human breast tumor cells in the PAM50 genes

ABSTRACT: Quantitative reverse transcription PCR analysis of human breast tumor cells in the PAM50 genes

PROVIDER: PRJNA439202 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Quantitative reverse transcription PCR analysis of human breast tumor cells in the PAM50 genes

Project description:Human formalin-fixed paraffin-embedded breast tumor tissue (1-4 1.5mm punches) was processed to RNA using High Pure RNA Paraffin Kit (Roche Applied Science, Indianapolis, IN) for quantitative reverse transcription polymerase chain reaction (qRT-PCR).

2018-04-01 | GSE112063 | GEO

Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex Reverse Transcription-PCR

Project description:Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex Reverse Transcription-PCR

| PRJNA1176519 | ENA

Reverse Transcription quantitative PCR (RT-q PCR) analysis of osteogenic-related markers for osteogenic differentiation from human periodontal ligamen stem cells

Project description:Reverse Transcription quantitative PCR (RT-q PCR) analysis of osteogenic-related markers for osteogenic differentiation from human periodontal ligamen stem cells

| PRJNA936165 | ENA

Highly multiplexed single-cell quantitative PCR

Project description:Highly multiplexed single-cell quantitative PCR

| PRJNA398516 | ENA

Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex Reverse Transcription-PCR

Project description:Reverse transcription polymerase chain reaction (RT-PCR) has evolved as a widely used approach in biotechnology and molecular diagnostics. It represents a powerful tool for amplifying and analysing RNA molecules and has therefore found widespread applications in profiling gene expression, viral detection and the diagnosis of various diseases. Well‑established methodologies use viral reverse transcriptases (RTs) to transcribe RNA to cDNA and thermostable DNA polymerases (DNA pols) to amplify the resulting target sequence by PCR. This study reports on the development of novel Thermus aquaticus DNA polymerase I (Taq pol) variants that each are able to catalyse both steps simultaneously in a single tube without the need of viral RTs. In combination with their excellent thermostability (up to 95°C), the novel Taq pol variants are suitable for employment in dye- or probe-based RNA detection methods. Moreover, the herein reported Taq pol variants are the first DNA pols that are capable of performing multiplex detection of various RNA targets in a single tube with a single enzyme. Thus, discovery marks a significant advancement of current RT-PCR approaches and contributes simplifying and reducing costs in molecular diagnostics.

2025-07-21 | GSE280134 | GEO

Quantitative PCR versus metagenomics for monitoring antibiotic resistance genes: balancing high sensitivity and broad coverage

Project description:Quantitative PCR versus metagenomics

| PRJEB59119 | ENA

Reverse Transcription quantitative PCR (RT-q PCR) analysis of osteogenic-related markers for osteogenic differentiation from human periodontal ligamen stem cells

Project description:We pre-treated human periodontal ligament stem cells (hPDLSCs) under Iinterleukin-6 (IL-6) in osteogenic inductive medium (OM) on osteogenic differentiation potential and investigate the specific signaling pathway by clarifying with several antagonists (Dkk-1, SP600125, SB431542, and DAPT) and siRNA (siWnt2B, siWnt10B, and siWnt5A) to identify the differences in the expression pattern of osteogenic related markers by reverse transcription quantitative polymerase chain reaction (RT-qPCR).

2023-02-19 | GSE225547 | GEO

Quantitative PCR (qRT-PCR) analysis of micro RNA expression in mouse cells

Project description:Quantitative PCR (qRT-PCR) analysis of micro RNA expression in mouse cells

| PRJNA1010752 | ENA

Real-time quantitative PCR analysis of microRNA in keratinocytes and reconstituted epidermis

Project description:Analysis of microRNA expression in keratinocytes by relative quantification (RQ) using the comparative Ct method. Real-time quantitative PCR analysis of microRNA in keratinocytes or reconstituted epidermis exposed to IL-22, LPS or UV radiation or a small molecule drug. Keratinocytes or reconstituted human epidermis were exposed to indicated treatments. A qPCR analysis of 365 microRNAs in keratinocytes. RNA (20 ng per reaction) reverse transcribed using 8 x Multiplex RT pools. Each resulting cDNA pool was mixed with TaqMan Universal PCR Master Mix and loaded in corresponding port on the TaqMan Array.

2013-05-23 | E-GEOD-41838 | biostudies-arrayexpress

Real-time quantitative PCR analysis of human exosomes

Project description:Real-time quantitative PCR analysis of human exosomes

| PRJNA504728 | ENA