Project description:Parallel Analysis of RNA Ends (PARE) sequencing reads were generated to validate putative microRNAs and identify cleavage sites in Sorghum bicolor and Setaria viridis.
Project description:The study is done to compare the impact of high light on the photosynthetic bacterium Rhodobacter alkalitolerans strain JA916T when grown in high pH and neutral pH. After the analysis we found several photosynthetic and cell division transcripts to be differentially expressed. These transcripts were majorly from the bacteriochlorophyll and carotenoid biosynthesis pathway. Reaction center transcripts were also found to be highly upregulated in nhpH conditions. Similary cell division transcripts were also found to be highly upregulated in high light conditions. Further for detailed study the publication can be referred. This study is to be published in the article titled as " Integration of transcriptomics and metabolomics for understanding the global responses to neutral pH and high pH under high light in photosynthetic bacterium Rhodobacter alkalitolerans strain JA916T".
Project description:mRNA levels were measured in Rhodobacter sphaeroides 2.4.1 at 20% O2 and 0.5% O2, Rhodobacter sphaeroides 2.4.1 App11 (AppA-null), Rhodobacter sphaeroides 2.4.1 (pPNs) and PpsR mutant PPS2-4. The mRNA samples were prepared from cultures supplied with 20% O2, 1% CO2, and 79% N2, and grown in the dark to an OD of 0.18. The mRNA levels for each strain was measured three times. Keywords: repeat sample
Project description:Setaria viridis TPS1 was immunoprecipitated. The sample was separated by SDS-PAGE. The putative SvTPS1 band was sliced from the gel and analysed by mass spectrometry.