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Transcriptome profile in IEC4.1 cells following Cryptosporidium parvum infection

ABSTRACT: Transcriptome profile in IEC4.1 cells following Cryptosporidium parvum infection

PROVIDER: PRJNA445379 | ENA |

REPOSITORIES: ENA

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Transcriptome profile in IEC4.1 cells following Cryptosporidium parvum infection

Project description:Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children in developing countries. Infection by this parasite causes significant alterations in the gene expression profiles in infected host cells. This study aims to measure the genomic wide alterations in gene expression profiles in host intestinal epithelial cells following C. parvum infection. Mouse intestinal epithelial (IEC4.1) cells were grown to 80% confluence and exposed to C. parvum infection for 24h. Total RNA was collected for the genome-wide analysis. The Agilent SurePrint G3 mouse Gene Expression Microarray (G4852A) was used for the genome-wide analysis, which provides full coverage of genes and transcripts with the most up-to-date content, including mRNAs and lincRNAs (http://www.chem.agilent.com/store/en_US/Prod-G4852A/G4852A).

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Transcriptomic profile of IEC4.1 cells treated with an siRNA to lncRNA NR_126553 (Nostril) in response to Cryptosporidium parvum

Project description:This study is to investigate the potential impact of an lncRNA NR_126553 (Nostril) in murine intestinal epithelial cells (IEC4.1 cells) in response to Cryptosporidium parvum (C. parvum) infection. NR_126553 (Nostril) was knocked down by using a pool of gene specific siRNAs (SiNostril). Cells treated with a scramble non-specific siRNA were used as the control (siNegative control). After siRNA transfection 24h, cells were exposed to C. parvum infection for 24h. Then total RNA was collected for sequencing via BGISEQ-500 platform to obtain a comprehensive view of the transcriptome.

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Transcriptome profile in INT cells following Cryptosporidium parvum infection

Project description:Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children in developing countries. Certain parasite molecules can be delivered into host epithelial cells and may act as effector molecules for parasite intracellular development. This study aims to measure the impact of transfection of a parasite low-protein coding potential RNA transcript, Cdg7_FLc_0990, on the transcriptome profile in intestinal epithelial cells. Human intestinal epithelial INT (FHs 74 Int) cells were grown to 80% confluence and transfected with the Cdg7_FLc_0990 full-length or the empty vector for 48h. Total RNA was collected for the genome-wide analysis. RNA from INT cells following C. parvum infection for 48h and from cell of the non-infected control was also collected for the analysis. The Agilent SurePrint G3 Human Gene Expression Microarray (G4851B) was used for the genome-wide analysis, which provides full coverage of genes and transcripts with the most up-to-date content, including mRNAs and lincRNAs (http://www.chem.agilent.com/store/en_US/Prod-G4851B/G4851B)

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MicroRNA expression profile of HCT-8 in the early phase of Cryptosporidium parvum infection

Project description:Cryptosporidium parvum is an important zoonotic parasitic disease worldwide, but the molecular mechanisms of the host–parasite interaction are not fully understood. Noncoding microRNAs (miRNAs) are considered key regulators of parasitic diseases. Therefore, we used microarray, qPCR, and bioinformatic analyses to investigate the intestinal epithelial miRNA expression profile after Cryptosporidium parvum infection.Twenty miRNAs were differentially expressed after infection (four upregulated and 16 downregulated). Further analysis of the differentially expressed miRNAs revealed that many important cellular responses were triggered by Cryptosporidium parvum infection, including cell apoptosis and the inflammatory and immune responses.This study demonstrates for the first time that the miRNA expression profile of human intestinal epithelium cells is altered by C. parvum infection. This dysregulation of miRNA expression may contribute to the regulation of host biological processes in response to C. parvum infection, including cell apoptosis and the immune responses. These results provide new insight into the regulatory mechanisms of host miRNAs during cryptosporidiosis, which may offer potential targets for future C. parvum control strategies.

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