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Transcriptome Mapping of Internal N7-methylguanosine Methylome in Mammalian mRNA

ABSTRACT: Transcriptome Mapping of Internal N7-methylguanosine Methylome in Mammalian mRNA

PROVIDER: PRJNA445453 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6904850.fastq.gz	Fastqsanger.gz
	SRR6904860.fastq.gz	Fastqsanger.gz
	SRR6904851.fastq.gz	Fastqsanger.gz
	SRR6904861.fastq.gz	Fastqsanger.gz
	SRR6904852.fastq.gz	Fastqsanger.gz

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Transcriptome Mapping of Internal N7-methylguanosine Methylome in Mammalian mRNA

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Project description:Previous study found the presence of internal N7-methylguanosine (m7G) within mammalian mRNA. We performed antibody-based m7G MeRIP-seq for transcriptome-wide mapping the internal m7G sites in U2OS cells.

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Project description:The project aims to identify the RNA binding proteins that can specifically bind to mRNA internal modification N7-methylguanosine (m7G). N7-methylguanosine (m7G) methylation has been long identified as the essential cap modification for eukaryotic mRNA, but later has been identified internally in various types of RNAs, including tRNA, rRNA, miRNA, and mRNA. While the METTL1/WDR4 complex, responsible for tRNA m7G installation, has been suggested to be responsible for internal mRNA modification as well, little is known about the proteins that can identify and interact with m7G. To better understand its interaction with RNA binding proteins and further define its regulatory function in mRNA metabolism, we performed the experiment to pulldown proteins with different binding affinity on unmodified G and m7G and subject the enriched fractions to mass spectrometry.

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Project description:Recent studies reported that N7-methylguanosine (m7G) modification exists in internal mRNAs; however, the “reader” protein for mRNA internal m7G modification is still unrevealed. Here, by performing m7G MeRIP-seq and RIP-seq, we identified quaking protein (QKI) as a novel internal m7G modification reader. Internal mRNA m7G modification acts as a key player in mRNA translation under stress condition. To explore the mechanism underlying the function of QKI in translation regulation, we employed RNA-seq and Ribo-seq, and demonstrated that QKI7 regulates the translation efficiency of a subset of internal m7G-modified transcripts under stress condition.

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Project description:Transcriptome Mapping of Internal N7-methylguanosine modification via m7G MeRIP-seq [m7g.1]

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