Project description:We report evidence that FZD2 is a driver for EMT, cancer stem cell (CSC) property, and vasculogenic mimicry (VM) in hepatocellular carcinoma. To further examine the transcriptional changes in response to FZD2 depletion, we performed RNA-seq with FZD2 knockdown and control SNU449 cells by BGISEQ-500.
Project description:We report the Phosphoserine Phosphatase (PSPH) would impair influence the Chemokines expression in TNF-A condition, Gene enrichment analysis of the transcriptome profiles of shPSPH-transfected hepatoma cells or shPLKO-transfected control cells could discover which changes in signaling pathways affect chemokine expression.PLC/PRF/5 and SNU449 were stimulated with human recombinant TNFα for 24 hours. RNA of the cells was extracted by TRIzol method.