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Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi Ring1a/b dKO]

ABSTRACT: Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi Ring1a/b dKO]

PROVIDER: PRJNA445861 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6904171.fastq.gz	Fastqsanger.gz
	SRR6904172.fastq.gz	Fastqsanger.gz
	SRR6904173.fastq.gz	Fastqsanger.gz
	SRR6904174.fastq.gz	Fastqsanger.gz
	SRR6904175.fastq.gz	Fastqsanger.gz

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Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi Ring1a/b dKO]

Project description:Polycomb repressive complexes (PRC) 1 and 2 are essential chromatin regulators of cell identity. PRC1, a dominant executer of Polycomb-mediated control, functions as multiple sub-complexes that possess catalytic-dependent H2AK119 mono-ubiquitination (H2AK119ub) and catalytic-independent activities. Here, we show that despite its well-established repressor functions, PRC1 binds to both silent and active genes. Through in vivo loss-of-function studies, we show that global PRC1 function is essential for skin development and stem cell (SC) specification.

2018-03-31 | GSE112382 | GEO

Promoter Capture-HiC in Ring1a KO and Ring1a/b dKO mouse ES cells

Project description:Genome organization influences transcriptional regulation by facilitating interactions between gene promoters and distal regulatory elements. To analyse distal promoter contacts mediated by the PRC1 complex we used Capture Hi-C (CHi-C) to enrich for promoter-interactions in a HiC library in Ring1a KO and Ring1a/b dKO mouse ES cells.

2015-08-01 | E-MTAB-3109 | biostudies-arrayexpress

Disruption of PRC1 Components RING1A and RING1B Promotes Angiogenesis via Relieving BMP4 Repression [RNA-Seq]

Project description:Angiogenesis is crucial for maintaining tissue homeostasis and responding to injury and disease. Epigenetic mechanisms have been elucidated to regulate the expression of pro-angiogenic factors and modulate angiogenesis. The Polycomb repressive complex 1 (PRC1) is a key epigenetic repressive complex that regulates multiple biological functions. Within this complex, RING1A and RING1B, which serve as the E3 ubiquitin ligases for PRC1, contribute to the monoubiquitylation of histone H2A on Lysine 119 (H2AK119ub), thereby repressing gene transcription. However, the role of RING1A and RING1B in regulating angiogenesis has remained elusive. In this study, we demonstrate that RING1A and RING1B suppress angiogenic capacity in vitro, although they do not inhibit migration and proliferation in endothelial cells (ECs). Furthermore, by integrating RNA sequencing data from RING1A knockdown or RING1B knockdown ECs with CUT&Tag assay results for RING1A, RING1B, and H2AK119ub in ECs, we have identified that PRC1 inhibits angiogenesis by repressing BMP4 expression, a pro-angiogenic factor known to regulate angiogenesis. Additionally, we verify that the knockdown of RING1A and RING1B enhances angiogenesis in vivo, which may provide a new therapeutic target for angiogenesis-related diseases.

2025-07-24 | GSE281872 | GEO

Disruption of PRC1 Components RING1A and RING1B Promotes Angiogenesis via Relieving BMP4 Repression [CUT&Tag]

2025-07-24 | GSE281870 | GEO

Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi Eed cKO]

Project description:Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi Eed cKO]

| PRJNA446059 | ENA

Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi ChIP-seq]

Project description:Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi ChIP-seq]

| PRJNA446062 | ENA

Promoter Capture-HiC in Ring1a KO and Ring1a/b dKO mouse ES cells

Project description:Promoter Capture-HiC in Ring1a KO and Ring1a/b dKO mouse ES cells

| PRJEB7794 | ENA

Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi Eed cKO]

2018-03-31 | GSE112402 | GEO

Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi ChIP-seq]

2018-03-31 | GSE112403 | GEO

Transcription profiling of mouse Ring1B single and Ring1A/B double knockout cells shows Ring1B interacts with Oct4.

Project description:The Polycomb group (PcG) gene products mediate heritable silencing of developmental regulators in metazoans, participating in one of two distinct multimeric protein complexes, the Polycomb repressive complexes-1 (PRC1) and -2 (PRC2)1-5. PRC2 catalyses trimethylation of histone H3 at lysine 27 (H3K27) which in turn is thought to provide a recruitment site for PRC13-7. Recent studies demonstrate that mono-ubiquitylation of histone H2A at lysine 119 is important in PcG mediated silencing with the core PRC1 component Ring1A/B functioning as the E3 ligase8. PRC2 has been shown to share target genes with the core transcription network to maintain embryonic stem (ES) cells including Oct4 and Nanog9. Here we identify an essential role for PRC1 in repressing developmental regulators in ES cells, and thereby in maintaining ES cell pluripotency. A significant proportion of the PRC1 target genes are also repressed by Oct4. We demonstrate that engagement of PRC1 and PRC2 at target genes is Oct4-dependent and moreover that Ring1B interacts with Oct4. Collectively these results show that PcG complexes are instrumental in Oct4-dependent repression required to maintain pluripotency of ES cells. This study provides a first functional link between a core ES cell regulator and global epigenetic regulation of the genome. Experiment Overall Design: We observed gene expression of Ring1B single and Ring1A/B double KO cells using Affymetrix chip: MOE 430 2.0. Experiment Overall Design: Because constitutive Ring1A/B-dKO cells can not be maintained we generated conditional KO cells with OHT.

2008-04-07 | E-GEOD-10476 | biostudies-arrayexpress

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