Oncorhynchus mykiss
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ABSTRACT:
PROVIDER: PRJNA448020 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR6956993_1.fastq.gz | Fastqsanger.gz | |||
| SRR6956993_2.fastq.gz | Fastqsanger.gz | |||
| SRR6956994_1.fastq.gz | Fastqsanger.gz | |||
| SRR6956994_2.fastq.gz | Fastqsanger.gz | |||
| SRR6956995_1.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Purpose:Our data significantly advance understanding of heat stress regulatory mechanism of miRNA in the head kidney of rainbow trout Methods:miRNAs of rainbow trout were involved in heat stress were identified by high-throughput sequencing of six small RNA libraries of the kidney tissues under control (18℃) and heat-treated (24℃) conditions Results:high-throughput sequencing was performed to identify miRNAs responsive to heat stress. We obtained 41,991,119 and 43,882,123 raw reads and 39,756,736 and 42,538,331 clean reads from under control (18℃) and heat-treated (24℃) .A total of 392 conserved miRNAs and 989 novel miRNAs were identified, of which 78 miRNAs were expressed in different response to heat stress. In addition to, including 393 negative correlation miRNA-target gene pairs Conclusions:through high-throughput sequencing of the six libraries from head kidney tissue of rainbow trout, the expression level of miRNA has significant changes after heat stress.
2018-03-16 | GSE111816 | GEO
Project description:Transcriptome profiling of rainbow trout liver under moderate heat stress
| PRJNA352601 | ENA
Project description:Hypoxia has emerged as a critical threat to the stability of aquatic ecosystems and the sustainability of aquaculture industries. Rainbow trout (Oncorhynchus mykiss), a globally significant economic fish species, exhibits remarkable sensitivity to hypoxia. However, the physiological mechanisms of head kidney in rainbow trout under hypoxia stress, especially under acute and severe hypoxia conditions, remain largely unexplored. This research explored the transcriptomic responses of rainbow trout head kidney to moderate hypoxia (4.5 ± 0.1 mg/L) and severe hypoxia (3.0 ± 0.1 mg/L) for 12 h, alongside dynamic changes in biochemical parameters during hypoxia exposure (0, 4, 8, 12, 24 h, and 1 month) and reoxygenation (R12 h and R24 h). The results showed that compared with the control group, the activities of T-AOC, CAT, T-SOD, GOT, GPT, and ACP were decreased under moderate and severe hypoxia conditions, while the content of MDA and the activity of AKP were increased. In RNA-seq analysis, we identified several hypoxia-related, immune-related and metabolism-related genes (hif-1α, fih1, vhl, ddit4, egln1/2/3, cxcr1/3/4/5, ccr5/7, clec4e/m, tlr2, socs2/3, ighv3-43, ighv3-53, g6pc3, pk, ldha, hk2, and foxo1a) from 20,399 significantly differentially expressed genes, which were enriched in insulin signaling pathway, glycolysis/gluconeogenesis, FcγR-mediated phagocytosis, hematopoietic cell lineage and B cell receptor signaling pathway. Additionally, the expression levels of cxcr1, ddit4, egln2, egln3, fih1 and foxo1a in rainbow trout were significantly up-regulated under hypoxia stress and returned to normal after reoxygenation. These findings provide new insights into the physiological and molecular responses of rainbow trout under hypoxia stress and contribute to developing effective strategies for fish to cope with environmental hypoxia.
2025-09-11 | GSE306836 | GEO
Project description:Rainbow trout is a typical cold-water fish, with the intensification of global warming, high temperatures severely restrict the development of aquaculture in summer. Understanding the molecular regulation mechanisms of rainbow trout in response to heat stress will be salutary to alleviate heat stress-related damage. In the present study, we performed transcriptome analysis of liver tissues in rainbow trout under heat stress (24℃) and control (18℃) conditions to identify induced lncRNAs and pathways by heat stress. More than 658 million clean reads and 5,916 lncRNAs were identified from six liver libraries. A total of 927 novel lncRNAs were generated and 428 differentially expressed lncRNAs were screened through stringent thresholds. The RNA-seq results were verified by RT-qPCR. In addition, the regulatory network of important functional lncRNA-mRNA were constructed. GO and KEGG enrichment analysis of target gene of differentially expressed lncRNAs were performed. Many target genes involved in maintaining homeostasis or adapting to stress and stimuli were highly induced under heat stress. Several important regulatory pathways were involved in heat stress, including thyroid hormone signaling pathway, PI3K-Akt signaling pathway, estrogen signaling pathway, etc. This result broadens our understanding of lncRNA associated with heat stress and provides new insights into lncRNA-mediated regulation of rainbow trout heat stress.
2019-08-11 | GSE135668 | GEO
Project description:Rainbow trout (Oncorhynchus mykiss) is a typical cold-water fish, the development of rainbow trout aquaculture was severely hampered via the high temperature in summer. Understanding the regulatory mechanism of rainbow trout response to chronic heat stress can provide a theoretical basis for formulating measures to relieve heat stress. In the study, changes in the biochemical parameters revealed that a strong stress response occurred in rainbow trout at 24 °C, the organisms stress defense system was activated, and the immune system was also affected. Proteome of rainbow trout liver tissues under heat stress (24 °C) and control conditions (18 °C) were performed using DIA/SWATH. A total of 390 DEPs were identified by strict threshold (q-value <0.05 and fold changes >1.5), among them 175 were up-regulated and 225 were down-regulated. Some proteins related to HSP, metabolism and immunity were identified. GO analysis showed that some proteins that were highly induced to express at high temperature were involved in the regulation of cell homeostasis, metabolism, adaptive stress and stimulation. KEGG analysis shows that some pathways play an important role in the regulation of heat stress, such as metabolic pathway, protein processing in endoplasmic reticulum pathway, PPAR signaling pathway and complement and coagulation cascades pathway, etc. PPI network analysis shows HSP90b1 and C3 maybe cooperative to protect the integrity of cell membrane function under heat stress. Our finding provide a comprehensive review of protein expression of rainbow trout liver under heat stress, which helps to formulate strategies for rainbow trout to relieve heat stress during high temperature in summer.
2020-07-06 | PXD020206 |
Project description:Rainbow trout (Oncorhynchus mykiss) is highly sensitive to high-temperature stress as an important economic cold-water fish. While previous research has concentrated on the transcriptomic to acute heat stress in rainbow trout, there remains a gap in knowledge regarding the overarching regulatory mechanisms at the translation level. In our research, we utilized a combination of transcriptomic and translatomic analyses to investigate the intricate molecular response mechanisms in the liver of rainbow trout when subjected to heat stress. Through comprehensive multi-omics analysis, we revealed the dynamic translational pattern of rainbow trout liver under heat stress for the first time. Comparative analysis of ribosome analysis data with RNA-seq data showed that the fold changes of gene expression at the transcriptional level were highly correlated (R2 = 0.83) with those at the translational level globally. In total, 2,203 genes exhibited significant alterations exclusively within the translational level. However, the limited overlap in response genes between transcription and translation under heat stress suggests that these two processes may independently modulate the cellular response to thermal challenges. Significant changes in the translation efficiency of 809 genes were observed under heat stress. Further analysis indicated that the translation efficiency of genes were strongly influenced by sequence characteristics such as GC content, coding sequence length and NMFE. Moreover, 3,468 putative uORFs were identified in 2,676 genes, which potentially modulating translation efficiency of mORFs. These findings provide a novel perspective for understanding the physiological adaptations of rainbow trout in response to changes in ambient temperature.
2024-04-01 | GSE262612 | GEO
Project description:The objective of this study was to identify and quantify proteomic profiles of head kidney of rainbow trout Oncorhynchus mykiss. Specific pathogen free rainbow trout (mean length 15 ± 1 cm) were maintained in recirculating de-chlorinated water at 19±1 °C. Prior to the experiment, fish were distributed between 9 aquaria, 18 fish per aquarium. The test groups were infected by immersion of Yersinia ruckeri strains: CSF007-82 (biotype 1) and 7959-11 (biotype 2). The control group was immersed similar with sterile broth medium. There were 3 aquaria per each group (CSF007-82-infected, 7959-11-infected and control). Nine fish from infected and control fish groups were anaesthetized with MS-222 at 3, 9 and 28 days post exposure and sampled aseptically. Each head kidney was washed three times with sterile phosphate-buffered saline containing a cocktail of mammalian protease inhibitors. Head kidney samples were snap-frozen in liquid nitrogen and stored at –80 °C.
2018-08-14 | PXD008473 | Pride
Project description:Rainbow trout (Oncorhynchus mykiss) is highly sensitive to high-temperature stress as an important economic cold-water fish. While previous research has concentrated on the transcriptomic to acute heat stress in rainbow trout, there remains a gap in knowledge regarding the overarching regulatory mechanisms at the translation level. In our research, we utilized a combination of transcriptomic and translatomic analyses to investigate the intricate molecular response mechanisms in the liver of rainbow trout when subjected to heat stress. Through comprehensive multi-omics analysis, we revealed the dynamic translational pattern of rainbow trout liver under heat stress for the first time. Comparative analysis of ribosome analysis data with RNA-seq data showed that the fold changes of gene expression at the transcriptional level were highly correlated (R2 = 0.83) with those at the translational level globally. In total, 2,203 genes exhibited significant alterations exclusively within the translational level. However, the limited overlap in response genes between transcription and translation under heat stress suggests that these two processes may independently modulate the cellular response to thermal challenges. Significant changes in the translation efficiency of 809 genes were observed under heat stress. Further analysis indicated that the translation efficiency of genes were strongly influenced by sequence characteristics such as GC content, coding sequence length and NMFE. Moreover, 3,468 putative uORFs were identified in 2,676 genes, which potentially modulating translation efficiency of mORFs. These findings provide a novel perspective for understanding the physiological adaptations of rainbow trout in response to changes in ambient temperature.
2024-04-16 | GSE263547 | GEO
Project description:With the intensification of global warming, rainbow trout is suffering from varying degrees thermal stimulation, heat stress may cause pathological signs or diseases by reducing the immune roles and then lead to mass mortality, so high temperatures severely restrict the development of its aquaculture. Understanding the molecular regulation mechanism of rainbow trout under heat stress is used to take measures to relieve symptoms. We performed multiple transcriptomic analysis of liver tissues from rainbow trout under heat stress (24 °C) and control conditions (18 °C) to identify circRNAs, miRNAs and mRNAs. Changes of non-specific immune parameters revealed that strong stress response of rainbow trout is caused in 24 °C. A total of 324 DEcircRNAs, 105 DEmiRNAs, and 1885 DEmRNAs were identified from six libraries, and ceRNA regulatory network is constructed. 301 circRNA–miRNA and 51 miRNA–mRNA negative correlation pairs were screened from ceRNA regulatory network, and predicted three regulatory correlation pairs that novel_circ_003889 - novel-m0674-3p - hsp90ab1, novel_circ_002325 - miR-18-y - HSPA13 and novel_circ_002446 - novel-m0556-3p - hsp70. Some genes involved in metabolic process, biological regulation or response to stimulus are highly induced at high temperatures. Several important pathways involved in heat stress were characterized, such as Protein processing in endoplasmic reticulum (ER), Estrogen signaling pathway, HIF-1 signaling pathway, etc. These results extend our understanding of the molecular mechanisms of heat stress response and expected to provide a novel insight into develop strategies for relieve heat stress.
2019-11-08 | GSE140112 | GEO
Project description:Hypoxia severely threatens the survival and growth of fish, thereby greatly restricting the development of aquaculture. Rainbow trout (Oncorhynchus mykiss) is highly sensitive to hypoxia, but the regulatory mechanism of rainbow trout spleen under different levels of hypoxia is not yet clear. In this study, we analyzed the effects of moderate hypoxia (DO: 4.5 ± 0.1 mg/L) and severe hypoxia (DO: 3.0 ± 0.1 mg/L) on the biochemical parameters of rainbow trout spleen at various time points (4, 8, 12, 24 h, 1 month, and re-oxygenation for 12 and 24 h). Additionally, we comparatively analyzed the transcriptomic changes in moderate and severe hypoxia at 12 h, as well as in normoxic controls. Antioxidant enzyme activities (T-AOC, T-SOD, GOT, GPT, CAT) rose markedly under both moderate and severe hypoxia, while MDA content peaked early and then declined. Immune-related enzymes (AKP, ACP) were elevated under moderate hypoxia but suppressed under severe hypoxia. Reoxygenation gradually restored the activities of antioxidant and immune enzymes, yet MDA surged again. Transcriptomic analysis identified 5766 DEGs. These DEGs were mainly enriched in the B cell receptor signaling, Glycolysis/Gluconeogenesis, Notch, MAPK, and PI3K-Akt pathways. The analysis of dynamic expression of hypoxia-associated genes revealed that hif-1a, acly, egln2, plpp3, fn1, and cd22 were upregulated under moderate hypoxia, whereas aldoa and ldha were downregulated, with their expression levels recovering during reoxygenation. egln1, egln2, egln3, aldoa, plpp3, pk, g6pc, and eno1a were upregulated during severe hypoxia stress, and their expression returned to baseline levels following reoxygenation. These results improve our comprehension of the molecular processes involved in hypoxia stress in rainbow trout.
2025-08-20 | GSE305539 | GEO