Project description:We used human foreskin fibroblast cells with CRISPR-Cas9 mediated knockout of RB1 and p130 (RBL2) (Control, sgRB1, sgP130, sgRB1+sg130) and measured gene expression changes after doxorubicin treatmnet (24 hr, 350 nM).
Project description:We used human foreskin fibroblast cells with CRISPR-Cas9 mediated knockout of RB1 and p130 (RBL2) (sgP130, sgRB1+sg130), knocked down p107 using siRNA and measured gene expression changes after doxorubicin treatmnet (24 hr, 350 nM).
Project description:Human fibroblasts infected with human cyotmegalovirus strain Towne at a multiplicity of infection equal to 6. Host transcriptional levels were quantitated at 12 and 96 hours post infection using Illumina HT-12 beadarrays. These array depostions are an extension of a previous submission, GSE50955.
Project description:The libraries contained in this experiment come from the whole cell fraction of independent growths of foreskin fibroblast cells. They are stranded PE76 Illumina GAIIx RNA-Seq libraries from rRNA-depleted Poly-A+ RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:The libraries contained in this experiment come from the whole cell fraction of independent growths of foreskin fibroblast cells. They are stranded PE76 Illumina GAIIx RNA-Seq libraries from rRNA-depleted and DSN normalized Poly-A- RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:Using RNA sequencing technology, we compared specific changes in the transcriptomic profiles in human foreskin fibroblast cells (HFF-1) following ORFV infection. ORFV specifically upregulated and downregulated a variety of genes, including genes involved in antiviral immune response, apoptosis, cell cycle and a series of signaling pathways, such as the IFN and p53-signaling pathways. A range of genes were downregulated at early stage. In contrast, the virus up-regulates a number of cellular genes at late stage.
Project description:We used microRNA microarrays (Affy miRNA 2.0 array) to compare the miRNA expression between proliferating (mock) and different time points (12, 48, 72 hours) of serum starvation (to induce quiescence) in Human Foreskin Fibroblast (HFF) cells.
Project description:There is scant data on the mechanisms through which asymptomatic STIs (aSTIs) alter risk of HIV acquisition in the male genital tract. Foreskin removal lowers the risk of HIV acquisition, but molecular events leading to this protection are unclear. Here we show that an asymptomatic urethral infection with Chlamydia trachomatis significantly alters the foreskin epithelial proteome composition. We developed and optimised a shotgun liquid chromatography coupled tandem mass spectrometry based proteomics approach and applied this to foreskins collected at the time of medical male circumcision from 16 aSTI+ cis-gender men and 10 age-matched STI- controls. We used a novel bioinformatic metaproteomic pipeline to detect differentially expressed proteins. Gene enrichment ontology analysis revealed proteins associated with inflammatory and immune activation function in both inner and outer foreskin from men with an aSTI. Neutrophil activation/degranulation and viral-evasion proteins were significantly enriched in foreskins from men with aSTI whereas homotypic cell-cell adhesion proteins were enriched in foreskin tissue from men without an STI. Collectively, our data show that an asymptomatic urethral sexually transmitted infection result in profound alterations in epitheial tissue that potentially causes depletion of barrier integrity and function and that may encourage HIV susceptibilty.