Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Halteria grandinella

ABSTRACT: Halteria grandinella macronuclear genome sequencing and assembly

PROVIDER: PRJNA448784 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR8270648_1.fastq.gz	Fastqsanger.gz
	SRR8270648_2.fastq.gz	Fastqsanger.gz
	SRR8282610_1.fastq.gz	Fastqsanger.gz
	SRR8282610_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 11

Similar Datasets

Genome-wide analysis of replication initiation in Tetrahymena [Vegetative growing cells]

Project description:We mapped Tetrahymena macronuclear replication origins on a genome-wide scale using nanopore sequencing. Nanopore sequencing quantifies individual BrdU incorporated replication intermediates on individual DNA molecules.

2025-08-08 | GSE210741 | GEO

Mating of the stichotrichous ciliate Oxytricha trifallax induces production of a class of 27nt small RNAs derived from the parental macronucleus

Project description:Here we report that soon after mating of Oxytricha trifallax, abundant 27nt small RNAs are produced that are not present prior to mating. We performed next generation sequencing of these 27nt RNAs from various times after mating. Using sequence comparisons between macronuclear and micronuclear versions of genes, we found that this 27nt RNA class derives from the parental macronucleus, not the developing macronucleus. These small RNAs are produced equally from both strands of macronuclear nanochromosomes, but in a highly non-uniform distribution along the length of the nanochromosome, and with a particular depletion in the 30 nt telomere-proximal positions. Unlike the Tetrahymena scanRNAs, the Oxytricha macronuclear-derived 27mers are not modified by 2'O-methylation at their 3' ends.

2012-04-19 | GSE37390 | GEO

Project description:Euplotes aediculatus macronuclear genome sequencing and assembly

| PRJNA903089 | ENA

Stylonychia MAC genome

Project description:Stylonychia lemnae macronuclear genome assembly

| PRJEB5806 | ENA

Genome-wide analysis of replication initiation in a TXR1 (Histone H3 K27 monomethyltranferase) overexpression strain in Tetrahymena

Project description:We mapped Tetrahymena macronuclear replication origins in TXR1 overexpression strain on a genome-wide scale using Okazaki fragments sequencing (OK-seq). Replication initiation sites of TXR1 overexpression strain followig cadmium induction of MMT1 promoter driven-TXR1 were studied compare to the uninduced TXR1 overexpression strain without cadmium using OK-seq.

2025-07-31 | GSE210493 | GEO

Mating of the stichotrichous ciliate Oxytricha trifallax induces production of a class of 27nt small RNAs derived from the parental macronucleus

2012-04-19 | E-GEOD-37390 | biostudies-arrayexpress

Biased transcription and selective degradation of small RNAs shape the pattern of DNA elimination in Tetrahymena

Project description:The ciliated protozoan Tetrahymena undergoes extensive programmed DNA elimination when the germline micronucleus produces the new macronucleus during sexual reproduction. DNA elimination is epigenetically controlled by DNA sequences of the parental macronuclear genome, and this epigenetic regulation is mediated by small RNAs (scnRNAs) of approximately 28-30 nucleotides that are produced and function by an RNAi-related mechanism. Here, we examine scnRNA production and turnover by deep sequencing. scnRNAs are produced exclusively from the micronucleus and non-homogeneously from a variety of chromosomal locations. scnRNAs are preferentially derived from the eliminated sequences, and this preference is mainly determined at the level of transcription. Despite this bias, a significant fraction of scnRNAs is also derived from the macronuclear-destined sequences, and these scnRNAs are degraded during the course of sexual reproduction. These results indicate that the pattern of DNA elimination in the new macronucleus is shaped by the biased transcription in the micronucleus and by the selective degradation of scnRNAs in the parental macronucleus.

2012-06-21 | GSE38768 | GEO

Genome-wide analysis of replication initiation in Tetrahymena

Project description:We mapped Tetrahymena macronuclear replication origins on a genome-wide scale using Okazaki fragments sequencing (OK-seq). OK-seq permits a high-resolution, quantitative analysis of replication fork initiation on a genome-wide scale that is dependent on the depth of coverage within a population of molecules. Replication initiation sites of the TXR1 (Histone H3 K27 monomethyltranferase) deletion strain were also studied compare to wild type using OK-seq.

2025-07-31 | GSE210487 | GEO

Fabrea salina

Project description:Sequencing and assembly of Fabrea salina macronuclear genome

| PRJNA822294 | ENA

Biased transcription and selective degradation of small RNAs shape the pattern of DNA elimination in Tetrahymena

2012-06-21 | E-GEOD-38768 | biostudies-arrayexpress