Ontology highlight
ABSTRACT:
PROVIDER: PRJNA448838 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR6945030_1.fastq.gz | Fastqsanger.gz | |||
| SRR6945030_2.fastq.gz | Fastqsanger.gz | |||
| SRR6945040_1.fastq.gz | Fastqsanger.gz | |||
| SRR6945040_2.fastq.gz | Fastqsanger.gz | |||
| SRR6945050_1.fastq.gz | Fastqsanger.gz |
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Project description:To study the responses of microbial communities to short-term nitrogen addition and warming,here we examine microbial communities in mangrove sediments subjected to a 4-months experimental simulation of eutrophication with 185 g m-2 year-1 nitrogen addition (N), 3oC warming (W) and nitrogen addition*warming interaction (NW).
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Project description:Functional redundancy in bacterial communities is expected to allow microbial assemblages to survive perturbation by allowing continuity in function despite compositional changes in communities. Recent evidence suggests, however, that microbial communities change both composition and function as a result of disturbance. We present evidence for a third response: resistance. We examined microbial community response to perturbation caused by nutrient enrichment in salt marsh sediments using deep pyrosequencing of 16S rRNA and functional gene microarrays targeting the nirS gene. Composition of the microbial community, as demonstrated by both genes, was unaffected by significant variations in external nutrient supply, despite demonstrable and diverse nutrient–induced changes in many aspects of marsh ecology. The lack of response to external forcing demonstrates a remarkable uncoupling between microbial composition and ecosystem-level biogeochemical processes and suggests that sediment microbial communities are able to resist some forms of perturbation.
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Project description:This project builds upon a DOE Subsurface Biogeochemical Research (SBR) project on the transport and metabolism of fresh, photosynthetically-derived (mainly periphyton) particulate organic matter (POM) in near-surface riverbed sediments at the PNNL SFA Hanford 300 Area study site. We propose to utilize a combination of Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and polar metabolomics, together with metagenomic/transcriptomic sequencing to track, in a substrate-explicit manner, the degradation of fresh POM in Hanford 300 Area riverbed sediments. The overarching hypothesis behind our proposed research is that there will be detectable chemical and (meta)genomic responses to the input of fresh POM. Many previous studies have demonstrated distinct metabolic and bulk geochemical responses to fresh POM input, and a few studies have directly examined the uptake and degradation of POM in permeable marine sediments. In addition, our recent studies at the Hanford 300 Area indicate that in situ input of fresh POM (likely dominated by periphyton detritus) to the near-surface riverbed is an important driver of both microbial community composition and activity in the vicinity of the Hanford 300 Area.
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