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Response by Fusarium oxysporum f.sp. lycopersici 4287 to 28 and 37 degrees celcius

ABSTRACT: Response by Fusarium oxysporum f.sp. lycopersici 4287 to 28 and 37 degrees celcius

PROVIDER: PRJNA450835 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7030093_1.fastq.gz	Fastqsanger.gz
	SRR7030093_2.fastq.gz	Fastqsanger.gz
	SRR7030094_1.fastq.gz	Fastqsanger.gz
	SRR7030094_2.fastq.gz	Fastqsanger.gz
	SRR7030095_1.fastq.gz	Fastqsanger.gz

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Response by Fusarium oxysporum f.sp. lycopersici 4287 to 28 and 37 degrees celcius

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Fusarium oxysporum f.sp. radicis-lycopersici induces distinct transcriptome reprogramming in resistant and susceptible isogenic tomato lines

Project description:Background: Fusarium oxysporum f.sp. radicis-lycopersici (FORL) is one of the most destructive necrotrophic pathogen of tomato, causing important field and greenhouse yield losses. Despite the relevant economic impact, little is known about the molecular mechanisms regulating the Fusarium oxysporum f.sp. radicis-lycopersici resistance in tomato. Results: A transcriptomic experiment was carried out in order to investigate major mechanisms of FORL response in resistant and susceptible isogenic tomato lines. The microarray analysis at 15 DPI reveals a distinct gene expression pattern between the two genotypes in the inoculated vs not inoculated conditions, furthermore confirmed by a q-PCR experiment. A model of plant response for both compatible and incompatible reaction was proposed. In particular, in the incompatible interaction an activation of defense genes related to secondary metabolites production and tryptophan metabolism was observed. Moreover, the cell osmotic potential maintenance after the FORL roots challenging was mediated by a dehydration induced protein. As for the compatible interaction the activation of an oxidative burst mediated by Peroxidases and a Cytochrome monooxigenase, conducedthe cells to degeneration and necrosis. Conclusions: Our work allowed a comprehensive understanding of the molecular basis of the tomato-FORL interaction. Result obtained emphasizes a different transcriptional reaction between the resistant and the susceptible genotype to FORL challenging. Our findings could lead to the improvement of strategies of this disease control.

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The comparison of gene expression profiles in hepatic stellate cells isolated from mouse livers by perfusion method at 37 degrees or 6 degrees

Project description:We compared transcriptome profiling (bulk RNA-seq) of hepatic stellate cells isolated from mouse livers by perfusion method using collagenase at 37 degrees or 6 degrees and evaluated the heat-stressed gene expression at 37 degrees.

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Tomato xylem sap proteome composition following endophyte- and R-gene mediated resistance towards Fusarium oxysporum f.sp. lycopersici inoculation.

Project description:Xylem sap proteome studies on susceptible or resistant tomato (Solanum lycopersicum) inoculated with endophytic and/or pathogenic strains of Fusarium oxysporum f.sp. lycopersici were conducted to get insights into the molecular differences between endophyte- and R-gene-mediated resistance (EMR and RMR). The EMR and RMR proteomes were compared to each other and to the mock control. Interestingly, specific PR-5 isoforms were found to exclusively accumulate during endophyte or genetic resistance, providing excellent markers to distinguish both resistance types at the molecular level.

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The expression profiles of log-phase and stationary-phase cells of Shigella flexneri grown at 30 degrees and 37 degrees Celsius

Project description:To obtain a global view of the response of S. flexneri at the expression level induced by temperature up-shift, we compared the expression profiles of log-phase and stationary-phase cells grown at 30 degrees and 37 degrees Celsius.

2010-06-02 | GSE16535 | GEO

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