Project description:This study aimed to characterise the transcriptomic response of the lactate-utilizing bacteria, Coprococcus catus and Anaerobutyricum soehngenii, grown on varying carbon sources. This work has allowed for identification of divergent gene clusters in each species contributing to the lactate utilisation pathway.
Project description:We report a mass spectrometry-based proteomics approach to advance the study of the feline proteome. We analyzed immortalized feline cells, various tissues and plasma using data-dependent acquisition (DDA) on a Q Exactive HF mass spectrometer to develop a Felis Catus PeptideAtlas from 157 acquired runs. Data were analyzed using the Trans-Proteomic-Pipeline. We report in the first PeptideAtlas of the domestic cat the identification of 242,844 unique peptides representing 8,972 feline proteins or 45.6% of the predicted UniProtKB Felis catus proteome based on 19,655 unique genes and their representative protein sequences.
