Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chaetomium fimeti

ABSTRACT: Chaetomium fimeti overview

PROVIDER: PRJNA464109 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR7141610_subreads.fastq.gz	Fastqsanger.gz
	SRR7141611_subreads.fastq.gz	Fastqsanger.gz
	SRR7141612_subreads.fastq.gz	Fastqsanger.gz
	SRR7141613_subreads.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Chaetomium

Project description:Chaetomium sp. overview

| PRJNA713448 | ENA

Chaetomium cochliodes

Project description:Chaetomium cochliodes overview

| PRJNA839836 | ENA

Chaetomium

Project description:Chaetomium Genome sequencing

| PRJNA937296 | ENA

Chaetomium globosum

Project description:Chaetomium globosum genome sequencing

| PRJNA1219539 | ENA

Chaetomium madrasense

Project description:Chaetomium madrasense Raw sequence reads

| PRJNA1204283 | ENA

Chaetomium sp. 1176438

Project description:Chaetomium sp. 1176438 Standard Draft genome sequencing

| PRJNA713184 | ENA

Chaetomium ovatoascomatis isolate:PP2

Project description:Chaetomium ovatoascomatis isolate:PP2 Genome sequencing

| PRJNA793734 | ENA

Chaetomium globosum strain:61239

Project description:Chaetomium globosum strain:61239 Genome sequencing

| PRJNA863430 | ENA

Identification and characterization of sugar regulated promoters in Chaetomium thermophilum

Project description:The thermophilic fungus Chaetomium thermophilum has been successfully used in the past for biochemical and high resolution structural studies of protein complexes, but subsequent functional analysis of these assemblies were hindered due to the lack of genetic tools in this thermophile, which are typically amenable in several other mesophilic eukaryotic model organisms, in particular the yeast Saccharomycers cerevisiae. Hence, we aimed to develop a regulatable gene-expression system in C. thermophilum, which might facilitate such in vivo studies, based on what we know about the galactose-inducible GAL promoter in yeast. To identify sugar-regulatable promoters in C. thermophilum, we performed comparative xylose- versus glucose-dependent gene expression studies, which uncovered a number of enzymes induced by xylose but repressed by glucose. Subsequently, we cloned the promoters of the two most stringently regulated genes, the xylosidase-like gene (XYL) and xylitol dehydrogenase (XDH), obtained from this genome-wide analysis in front of the thermostable YFP (yellow fluorescent protein) reporter. In this way, we could demonstrate xylose-dependent YFP expression by either western blotting or life cell imaging fluorescence microscopy. Prompted by these results, we finally expressed a well-characterized dominant-negative ribosome assembly factor mutant, rsa4 E117>D, under the control of the XDH promoter, which allowed us to induce a nuclear export defect of the pre-60S subunit when C. thermophilum cells were grown in xylose but not glucose containing medium. Altogether, our study recognized xylose-regulatable promoters in Chaetomium thermophilum, which may foster functional studies of genes of interest in this thermophilic eukaryotic model organism.

2022-09-26 | GSE214043 | GEO