Project description:Gene expression level of Clostridioides difficile (C. difficile) strain R20291 comparing control C. difficile carring pMTL84151 as vector plasmid with C. difficile conjugated with a pMTL84151-03890 gene. Goal was to determine the effects of 03890 gene conjugation on C. difficile strain R20291 gene expression.
Project description:To reveal the effects of deleting the Δxtr gene in Clostridioides difficile, we first constructed a Δxtr knockout mutant and then examined the resulting phenotypic changes. Finally, to elucidate the underlying mechanisms, we performed RNA-sequencing-based transcriptome analysis.