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CRAC of yeast RNA polymerase II in anchor-away and auxin-degron strains.

ABSTRACT: CRAC of yeast RNA polymerase II in anchor-away and auxin-degron strains.

PROVIDER: PRJNA471852 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7178203.fastq.gz	Fastqsanger.gz
	SRR7178204.fastq.gz	Fastqsanger.gz
	SRR7178205.fastq.gz	Fastqsanger.gz
	SRR7178206.fastq.gz	Fastqsanger.gz
	SRR7178207.fastq.gz	Fastqsanger.gz

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CRAC of yeast RNA polymerase II in anchor-away and auxin-degron strains.

Project description:We obtained RNA polymerase II occupancy profiles across the genome of S.cerevisiae strains: Abf1 anchor-away cells after addition of rapamycin for different time points (including no addition of rapamycin) and Rap1-AID auxin-degron cells after addition of auxin for different time points (including no addition of auxin) . This allowed us to compare polymerase occupancy profiles when these proteins are depleted from the nucleus or degraded.

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Project description:We obtained RNA polymerase II occupancy profile across the genome of S.cerevisiae in several strains, Wild type at 25 and 37 degrees C, RNA15-1 at 25 and 37 degrees C, anchor-away parental strain plus rapamycin, Reb1 anchor-away plus rapamycin, Rap1 anchor-away plus rapamycin, Nrd1-AID plus auxin, and nrd1-AID without auxin. This allowed us to compare polymerase profiles when these proteins are depleted from the nucleus.

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