Project description:Human Bocavirus 1(HBoV1), which belongs to the genus Bocaparvovirus of the family Parvoviridae, infects well differentiated human airway epithelium which is at mitotically quiescent state. To systematicaly investigate the interaction between HBoV1 and primary human airway epithelium cultured at an air-liquid interface (HAE-ALI), RNA-seq was applied to study the transcriptome profile of HAE-ALI infected by HBoV1.
Project description:Human Bocavirus 1(HBoV1), which belongs to the genus Bocaparvovirus of the family Parvoviridae, infects well differentiated human airway epithelium which is at mitotically quiescent state. To systematicaly investigate the host and viral small RNA expression after HBoV1 infection of primary human airway epithelium cultured at an air-liquid interface (HAE-ALI), small RNA-seq was applied to study the small RNA transcriptome profile of HAE-ALI infected by HBoV1.
Project description:Human bocavirus (HBoV) is a newly discovered parvovirus identified from pooled nasopharyngeal aspirate specimens. Human bocavirus 1 (HBoV1) is a respiratory virus observed in respiratory samples from small children presenting bronchiolitis, wheezing, cough, fever, and rhinorrhea. It is the fourth most common virus detected in respiratory infections. DNA of HBoV1 was detected in up to 18% of nasal or nasopharyngeal samples and another study has been shown that over than 85% of children in the United States have antibodies to this virus. HBoV1 is a small DNA virus with a nonenveloped icosahedral capsid. This virus previously has been associated with wheezing, acute otitis media, severe pneumonia and respiratory failure. HBoV 1 has been also detected in the blood of acute respiratory patients and the selected group of immunocompromised children and also determined in healthy blood donors. The role of HBoV1 in the inflammatory process is poorly known. The aim of this project is to clarify the role of HBOV1 in the immunoregulatory mechanisms.
Project description:Human bocavirus 1 (HBoV1) is a common cause of respiratory tract infection in children. It replicates efficiently in human airway epithelial (HAE) cells and is shed into the airways for months after acute infection. It further persists in B cells of tonsillar germinal centers with unknown consequences. Our aim was to compare how acute and persistent virus infections influence the cells. We performed total RNA sequencing to investigate the cellular transcriptomes of polarized HAE cells infected with HBoV1 at 7, 14 and 30 days post infection, and compared the biological pathways enriched across these different time points. We observed significant activation in the interferon signaling pathway and elevation of pattern recognition receptors, including toll-like receptor 2, only in the acute phase, whereas a slight elevation of the p53-signalling pathway was still observed after 30 days. The results indicate that the cells seem to reach an equilibrium state with the virus where viral activity and cellular impact are balanced.