Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell staging of meiotic entry identifies a sequential order in meiotic sex chromosome activation

ABSTRACT: Single-cell staging of meiotic entry identifies a sequential order in meiotic sex chromosome activation

PROVIDER: PRJNA472624 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7207120_1.fastq.gz	Fastqsanger.gz
	SRR7207120_2.fastq.gz	Fastqsanger.gz
	SRR7207111_1.fastq.gz	Fastqsanger.gz
	SRR7207111_2.fastq.gz	Fastqsanger.gz
	SRR7207121_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 22

Similar Datasets

Single-cell staging of meiotic entry identifies a sequential order in meiotic sex chromosome activation

Project description:The switch from mitosis to meiosis is a major transition that takes place during germ cell development. The precise sequence and the different gene expression programs activated during this process are only partly known. Here, we applied single-cell mRNA sequencing to interrogate the transcriptional changes that occur during the early steps of male germ cell differentiation. We isolated single cells from testes using a Dazl-GFP reporter mouse, which allowed us to focus on germ cells undergoing the mitotic to meiotic transition. We identified 4 distinct meiotic stages with unique transcriptome profiles and reconstructed the timeline of the meiotic entry in silico, from spermatogonia up to the pachytene stage, identifying transcriptional changes with an unprecedented resolution. This allowed us to characterize 3 major transitions in the meiotic prophase 1 of the male germline: meiotic entry, the meiotic sex chromosome inactivation (MSCI), and concomitant pachytene transcriptional activation. Meiotic entry is initiated following the downregulation of a tightly connected set of pluripotency factors and accompanied by a global transcriptional silencing. In contrast, during subsequent sex chromosome inactivation at the zygotene stage, gene silencing proceeds in a defined order, related to gene function.

2021-05-22 | GSE114788 | GEO

MAX controls meiotic entry in sexually undifferentiated germ cells

Project description:MAX controls meiotic entry in sexually undifferentiated germ cells

| PRJNA1022400 | ENA

Transcription factor NFYA directs male meiotic entry by facilitating accessible chromatin at the promoters of genes expressed during meiosis [CUT&RUN]

Project description:Meiotic prophase I characterized by homologous recombination and synapsis is an intricate step for spermatogenesis. This process entails extensive changes to chromatin and transcription. Recent studies have revealed that prior to prophase I, accessible chromatin bound by paused Pol II at meiotic gene promoters is essential for their timely activation during later stages of prophase I. However, the factor responsible for promoting accessible chromatin at meiotic gene promoters before entry into prophase I is unknown. Here, we discovered that NFYA expressed in pre-meiotic germ cells promotes accessible chromatin at meiotic gene promoters. Concordantly, conditional germline deletion of Nfya in male mice blocks meiotic entry. Functionally, our spatial and single-cell ATAC-seq data revealed that loss of NFYA in pre-meiotic cells disrupts accessible chromatin at meiotic gene promoters. Our study identifies a pioneer role for NFYA in facilitating permissive chromatin at meiotic gene promoters before meiosis, thereby controlling the timely activation of meiotic genetic program during later meiosis.

2025-05-10 | GSE295964 | GEO

Transcription factor NFYA directs male meiotic entry by facilitating accessible chromatin at the promoters of genes expressed during meiosis [ATAC-seq]

2025-05-10 | GSE295941 | GEO

Transcription factor NFYA directs male meiotic entry by facilitating accessible chromatin at the promoters of genes expressed during meiosis [scRNA-seq & scATAC-seq]

2025-05-10 | GSE296438 | GEO

Transcription factor NFYA directs male meiotic entry by facilitating accessible chromatin at meiotic promoters in mice

Project description:Transcription factor NFYA directs male meiotic entry by facilitating accessible chromatin at meiotic promoters in mice

| PRJNA1257271 | ENA

foxl2l regulates female meiotic entry during juvenile gonadal development in zebrafish

Project description:Sex determination is distinct from development of other organs for its diversity among species. In zebrafish, the sex determination in juvenile stage could be hermaphroditic or gonochoristic. However, the detail mechanism is still unclear. Here in this study, we found that the transcription factor that belongs to forkhead box family, Foxl2l, is required for determination of female fate in zebrafish. Foxl2l was expressed from larva stage and became female bias in juvenile stage. The expression was restricted in pre-meiotic germ cells. Disruption of Foxl2l function by CRISPR/Cas9 system resulted in all-male phenotype in the mutant. Germ cells in foxl2l mutant were arrested in pre-meiotic stage at 21 dpf, the critical period for female meiotic entry. To further verify the downstream mechanism of Foxl2l on female sex determination, wildtype or foxl2l mutant germ cells labeled by foxl2l:egfp at 21 days post fertilization (dpf) and germ cells labeled by ziwi:egfp at 26 dpf were analyzed by RNA-seq and single-cell RNA-seq respectively

2025-05-01 | GSE173480 | GEO

foxl2l regulates female meiotic entry during juvenile gonadal development in zebrafish

Project description:foxl2l regulates female meiotic entry during juvenile gonadal development in zebrafish

| PRJNA725806 | ENA

Sequential Waves of Protein Phosphorylation Functionally Rewire the Proteome to Drive Gametogenesis

Project description:Using phosphoproteomics, we show that regulated waves of protein phosphorylation affect virtually all cellular functions to supoort meiotic entry, meiotic progeression and gamete morphogenesis.

2022-12-13 | PXD033675 | Pride

LUTI-dependent Downregulation of a Cell Cycle Transcription Factor is Key for Timely Meiotic Entry

Project description:LUTI-dependent Downregulation of a Cell Cycle Transcription Factor is Key for Timely Meiotic Entry

| PRJNA938113 | ENA