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Arabidopsis TRM5 encodes a nuclear-localised tRNA guanine-N1-methyltransferase and is important for plant growth

ABSTRACT: Arabidopsis TRM5 encodes a nuclear-localised tRNA guanine-N1-methyltransferase and is important for plant growth

PROVIDER: PRJNA472997 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7216020_1.fastq.gz	Fastqsanger.gz
	SRR7216020_2.fastq.gz	Fastqsanger.gz
	SRR7216030_1.fastq.gz	Fastqsanger.gz
	SRR7216030_2.fastq.gz	Fastqsanger.gz
	SRR7216021_1.fastq.gz	Fastqsanger.gz

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Arabidopsis TRM5 encodes a nuclear-localised tRNA guanine-N1-methyltransferase and is important for plant growth

Project description:We show that trm5 plants have less m1G in tRNAs, a slower leaf initiation rate, delayed flowering, and reduced primary root length. Late flowering correlates with reduced transcription of floral activators CO, FT, SOC1 and AP1. We also show that TRM5 methylates tRNA position 37 of several nucleus-encoded tRNA species, trm5 plants have reduced m1G and TRM5 is localized to the nucleus. Our findings highlight the importance of the post-transcriptional tRNA modification m1G37 in plant growth and development.

2019-08-25 | GSE114898 | GEO

Arabidopsis TRM5 encodes a nuclear-localised bifunctional tRNA guanine and inosine-N1-methyltransferase that is important for growth.

Project description:Modified nucleosides in tRNAs are critical for protein translation. N1-methylguanosine-37 and N1-methylinosine-37 in tRNAs, both located at the 3'-adjacent to the anticodon, are formed by Trm5. Here we describe Arabidopsis thaliana AtTRM5 (At3g56120) as a Trm5 ortholog. Attrm5 mutant plants have overall slower growth as observed by slower leaf initiation rate, delayed flowering and reduced primary root length. In Attrm5 mutants, mRNAs of flowering time genes are less abundant and correlated with delayed flowering. We show that AtTRM5 complements the yeast trm5 mutant, and in vitro methylates tRNA guanosine-37 to produce N1-methylguanosine (m1G). We also show in vitro that AtTRM5 methylates tRNA inosine-37 to produce N1-methylinosine (m1I) and in Attrm5 mutant plants, we show a reduction of both N1-methylguanosine and N1-methylinosine. We also show that AtTRM5 is localized to the nucleus in plant cells. Proteomics data showed that photosynthetic protein abundance is affected in Attrm5 mutant plants. Finally, we show tRNA-Ala aminoacylation is not affected in Attrm5 mutants. However the abundance of tRNA-Ala and tRNA-Asp 5' half cleavage products are deduced. Our findings highlight the bifunctionality of AtTRM5 and the importance of the post-transcriptional tRNA modifications m1G and m1I at tRNA position 37 in general plant growth and development.

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