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ABSTRACT: Nile tilapia microbiome

PROVIDER: PRJNA473236 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7223460.fastq.gz	Fastqsanger.gz
	SRR7223470.fastq.gz	Fastqsanger.gz
	SRR7223480.fastq.gz	Fastqsanger.gz
	SRR7223461.fastq.gz	Fastqsanger.gz
	SRR7223471.fastq.gz	Fastqsanger.gz

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Project description:Nile tilapia larvae microbiome

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Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

Project description:In fish, the sex determining mechanisms can broadly be classified as genotypic (GSD), temperature-dependent (TSD), or genotypic plus temperature effects (GSD+TE). For the fish species with TSD or GSD+TE, extremely high or low temperature can affect its sex determination and differentiation. For long time, the underlying changes in DNA methylation that occur during high or low temperature induced sex reversal have not been fully clarified. In this study, we used Nile tilapia as a model to perform a genome-wide survey of differences in DNA methylation in female and male gonads between control and high temperature induced groups using methylated DNA immunoprecipitation (MeDIP). We identified the high temperature induction-related differentially methylated regions (DMRs), and performed functional enrichment analysis for genes exhibiting DMR. These identified differentially methylated genes were potentially involved in the connection between environmental temperature and sex reversal in Nile tilapia. In this study, four samples (control females, CF; control males, CM; induced females, IF; induced males, IM) were analyzed.

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Epigenetic mapping of the somatotropic axis reveals differential DNA hydroxymethylation marks with potential implication in growth

Project description:We report an association of DNA hydroxymethylation profiling at single nucleotide resolution with gene expression in the fast muscle of Nile tilapia.

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Project description:Oral microbiome of Nile tilapia

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Project description:Microbiome of Nile Tilapia gut

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Characterization of elongation of the very long chain fatty acid protein 6 (elovl6) gene in male Oreochromis niloticus and its potential regulatory roles in lipid and glucose metabolism

Project description:Elongation of very long-chain fatty acids protein 6 (Elovl6) is a crucial enzyme in the synthesis of endogenous fatty acids, which participates in the energy balance and metabolic diseases. The main objective of this study was to explore the molecular characterization of elovl6 and study regulatory mechanism of elovl6 on male Nile tilapia, Oreochromis niloticus. In the present study, the full-length cDNA of Nile tilapia elovl6 was 2255 bp, comprising a 5'-UTR of 193 bp, an ORF of 810 bp, and a 3'-UTR of 1252 bp, encoding 269 amino acids that possesses the characteristic features of Elovl proteins. The transcription expression of elovl6 transcripts showed differential expression in different tissues and fed with different lipid sources diet. Antisense RNA technology was used to knock-down elovl6 in Nile tilapia. Compared with the control treatments (transfected with ultrapure water (control) or the blank expression vector (negative control)), knockdown elovl6 altered hepatic morphology, Long chain fatty acid synthesis, fatty acid oxidation, lead to excess fat deposition in the liver, ultimately cause abnormal glucose/lipid metabolism in male Nile tilapia. Transcriptome analyses (AGPAT2 knock-down vs. negative control) revealed a total of 5877 differentially expressed genes (DEGs). Transcriptome analyses revealed DEGs were greatly involved in some signaling pathways, such as PPAR signaling pathway, fatty acid degradation, glycolysis/gluconeogenesis, and insulin signaling pathway, which were related to lipid and glucose metabolism. We used qRT-PCR to verify the mRNA expression changes of 13 DEGs in related signaling pathways. Our results demonstrate that knock-down of elovl6 in tilapia blocks the synthesis of oleic acid and leads to abnormal glucose/lipid metabolism.

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Development of gene expression signatures for the HDF and MDA-MB-231 cells that received TP4 (a cationic antimicrobial peptide) treatment

Project description:Triple-negative breast cancer (TNBC) lacks therapeutic target and is difficult to treat. We report a cationic antimicrobial peptide (CAP), tilapia piscidin 4 (TP4), derived from Nile tilapia (Oreochromis niloticus), selectively toxic to TNBC. Here we aim to identify potential target in TNBC cell response to TP4 treatment by microarray study and to further address the role of TP4-resposive genes involved in TNBC cell death.

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Project description:The gill microbiome of Nile tilapia

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Gut microbiome in Nile tilapia

Project description:Gut microbiome in Nile tilapia Metagenome

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