Alternaria sp. MG1 isolate:grape
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PROVIDER: PRJNA473621 | ENA |
REPOSITORIES: ENA
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Project description:Alternaria sp. MG1 Genome sequencing and assembly
| PRJNA416051 | ENA
Project description:Alternaria sp. MG1 Genome sequencing and assembly
| PRJNA417218 | ENA
Project description:Alternaria sp. MG1 Transcriptome or Gene expression
| PRJNA288914 | ENA
Project description:Meloidogyne graminicola isolate:MG1 Genome sequencing
| PRJNA541790 | ENA
Project description:Campylobacter sp. MG1 Genome sequencing and assembly
| PRJNA875047 | ENA
Project description:we performed a comparative proteomics analysis of Korla fragrant pear after inoculation with Alternaria sp., at 0h, 24h, 72h, 120 h using iTRAQ-based quantitative proteomic technique. This study aimed to investigate the protein species expression profiles in response to Alternaria sp., infection, explore the potential molecular mechanisms of Korla fragrant pear response against Alternaria sp., and further obtain a comprehensive understanding of Korla fragrant pear-Alternaria sp interactions. The finding may provide novel clues to Korla fragrant pear resistance to Alternaria sp., and may lay the foundation for an in-depth understanding of the interaction between Korla fragrant pear and Alternaria sp., at the proteome level.
2020-06-04 | PXD019558 |
Project description:It was previously shown that interferon defects in latently HIV infected cells make them permissive to MG1 mediated killing. To increase effectiveness of MG1 therapy, we have combined MG1 with the SMAC mimetic birinapant to increase MG1 mediated killing in latently infected J1.1 cells. Increased cell death was observed compared to either treatment alone and was accompanied by increased caspase-3/7 and caspase-1 expression. However, cell death could not be blocked by the pan-caspase inhibitor ZVAD-fmk or the necroptosis inhibitor necrostatin1-s, alone or in combination, indicating that cell death does not occur via a single pathway. Furthermore, it was shown that SMAC mimetic treatment results in decreased transcription of genes that take part in the immune signalling pathway, which is likely one the ways how SMAC mimetic treament senstizes HIV infected cells to MG1 mediated death.
2025-03-07 | GSE278469 | GEO
Project description:BackgroundAlternaria sp. MG1, an endophytic fungus isolated from grape, is a native producer of resveratrol, which has important application potential. However, the metabolic characteristics and physiological behavior of MG1 still remains mostly unraveled. In addition, the resveratrol production of the strain is low. Thus, the whole-genome sequencing is highly required for elucidating the resveratrol biosynthesis pathway. Furthermore, the metabolic network model of MG1 was constructed to provide a computational guided approach for improving the yield of resveratrol.ResultsFirstly, a draft genomic sequence of MG1 was generated with a size of 34.7 Mbp and a GC content of 50.96%. Genome annotation indicated that MG1 possessed complete biosynthesis pathways for stilbenoids, flavonoids, and lignins. Eight secondary metabolites involved in these pathways were detected by GC-MS analysis, confirming the metabolic diversity of MG1. Furthermore, the first genome-scale metabolic network of Alternaria sp. MG1 (named iYL1539) was reconstructed, accounting for 1539 genes, 2231 metabolites, and 2255 reactions. The model was validated qualitatively and quantitatively by comparing the in silico simulation with experimental data, and the results showed a high consistency. In iYL1539, 56 genes were identified as growth essential in rich medium. According to constraint-based analysis, the importance of cofactors for the resveratrol biosynthesis was successfully demonstrated. Ethanol addition was predicted in silico to be an effective method to improve resveratrol production by strengthening acetyl-CoA synthesis and pentose phosphate pathway, and was verified experimentally with a 26.31% increase of resveratrol. Finally, 6 genes were identified as potential targets for resveratrol over-production by the recently developed methodology. The target-genes were validated using salicylic acid as elicitor, leading to an increase of resveratrol yield by 33.32% and the expression of gene 4CL and CHS by 1.8- and 1.6-fold, respectively.ConclusionsThis study details the diverse capability and key genes of Alternaria sp. MG1 to produce multiple secondary metabolites. The first model of the species Alternaria was constructed, providing an overall understanding of the physiological behavior and metabolic characteristics of MG1. The model is a highly useful tool for enhancing productivity by rational design of the metabolic pathway for resveratrol and other secondary metabolites.
| S-EPMC6345013 | biostudies-literature