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Identifying Lsr2 binding sites in Streptomyces venezuelae

ABSTRACT: Identifying Lsr2 binding sites in Streptomyces venezuelae

PROVIDER: PRJNA475288 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7282570_1.fastq.gz	Fastqsanger.gz
	SRR7282570_2.fastq.gz	Fastqsanger.gz
	SRR7282567_1.fastq.gz	Fastqsanger.gz
	SRR7282567_2.fastq.gz	Fastqsanger.gz
	SRR7282568_1.fastq.gz	Fastqsanger.gz

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Identifying Lsr2 binding sites in Streptomyces venezuelae

Project description:The goal of this work was to determine where Lsr2 binds within the S. venezuelae chromosome, and to differentiate between direct versus indirect effects by comparing our ChIP-seq results with RNA-seq results

2018-06-09 | GSE115538 | GEO

LsrL modulates Lsr2-induced chromatin structure to tune biosynthetic gene cluster regulation in Streptomyces venezuelae [IPOD-HR]

Project description:Specialized biosynthetic gene clusters in Streptomyces are subject to complex regulation involving both transcriptional control and chromosome organization. The nucleoid-associated protein Lsr2 silences many of these clusters, yet how it shapes the global chromatin structure and how its conserved paralog LsrL contributes to this process remain poorly understood. In this study, we applied a multi-omics approach, combining transcriptional activity, genome-wide protein-DNA binding profiles, and three-dimensional chromosome conformation to characterize the coordination of Lsr2 and LsrL in exerting transcriptional control and genome architecture in Streptomyces venezuelae. In line with established Lsr2 functions, we find that Lsr2 sets broad transcriptional boundaries, while LsrL acts in a more context-specific manner that depends on the presence of Lsr2 and may function to reinforce or modulate Lsr2-mediated silencing. Loss of Lsr2 reshaped the chromatin landscape genome-wide, relieving its restriction on short-range contacts, triggering strong transcriptional changes and new domain boundaries near de-repressed BGCs. These findings establish Lsr2 as a dominant but contextually modulated regulator whose interplay with LsrL coordinates specialized metabolism with higher-order chromosome organization.

2026-02-11 | GSE310101 | GEO

LsrL modulates Lsr2-induced chromatin structure to tune biosynthetic gene cluster regulation in Streptomyces venezuelae [RNA-seq]

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LsrL modulates Lsr2-induced chromatin structure to tune biosynthetic gene cluster regulation in Streptomyces venezuelae [ChIP-seq]

2026-02-11 | GSE310104 | GEO

Gene expression of wild type and lsr2 mutants of Streptomyces venezuelae during late development in liquid culture

Project description:Comparing gene expression of wild type and lsr2 mutants, to determine the effect of Lsr2 on Streptomyces development and metabolism

2018-06-09 | GSE115537 | GEO

Lsr2 binding sites and gene expression of wild type and lsr2 mutants of Streptomyces venezuelae during late development in liquid culture

Project description:Lsr2 binding sites and gene expression of wild type and lsr2 mutants of Streptomyces venezuelae during late development in liquid culture

| PRJNA475286 | ENA

ChIP-chip analysis of GlnR-FLAG binding sites in Streptomyces venezuelae under varying conditions of nitrogen availibility

Project description:ChIP-chip analysis of GlnR-FLAG binding sites in Streptomyces venezuelae under varying conditions of nitrogen availibility

2011-04-20 | E-MEXP-2933 | biostudies-arrayexpress

ChIP-chip to determine bldN binding sites in Streptomyces venezuelae

Project description:ChIP-chip of genomic DNA of Streptomyces venezuelae using polyclonal antibody against bldN

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Identifying SigG1 binding sites in Streptomyces tsukubaensis

Project description:Identifying SigG1 binding sites in Streptomyces tsukubaensis

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Determination by ChIP-chip of the binding sites of the developmental transcription regulator BldC in the genome of Streptomyces venezuelae ATCC 10712

Project description:BldC is a transcriptional regulator essential for morphological development Streptomyces venezuelae. Although bldC deletion strain is unable to produce aerial hyphae, electron microscopy reveals that almost all of the colony biomass is in the form of spores rather than undifferentiated vegetative hyphae. This ChIP-chip experiment was carried out to determine the binding sites, and thence the regulon, of BldC in Streptomyces venezuelae. Cy3(IP):Cy5(Total) signal ratios in the wild type were compared to those in a bldC knockout strain.

2017-11-01 | E-MTAB-6129 | biostudies-arrayexpress

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