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ABSTRACT: Bacterial community of organic waste streams, black soldier fly larvae and residues

PROVIDER: PRJNA476046 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR7346010_1.fastq.gz	Fastqsanger.gz
	SRR7346010_2.fastq.gz	Fastqsanger.gz
	SRR7346020_1.fastq.gz	Fastqsanger.gz
	SRR7346020_2.fastq.gz	Fastqsanger.gz
	SRR7346030_1.fastq.gz	Fastqsanger.gz

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Project description:The larvae of black soldier fly (BSF) Hermetia illucens (Diptera: Stratiomyidae), has demonstrated ability in the efficient bioconversion of organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC-MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both BSF larvae body and gut. No specific protocol was superior in capturing the BSF body and gut proteome, but each yielded complementary information to improve BSF proteome coverage. Protocol-specific functional annotation using protein level information has shown that the selection of extraction buffer can affect protein detection and their associated functional classes within the measured BSF larval gut proteome. Metaproteome analysis on BSF larvae gut has uncovered the prevalence of two bacterial phyla: actinobacteria and proteobacteria. We envisage that comparing a range of extraction protocols and investigating the proteome from the BSF body and gut separately will expand the fundamental knowledge of the BSF proteome and thereby provide translational opportunities for future research to enhance their efficiency for waste degradation and contribution to the circular economy.

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