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Sex-specific gene expression differences are evident in human embryonic stem cells and during in vitro differentiation of human placental progenitor cells

ABSTRACT: Sex-specific gene expression differences are evident in human embryonic stem cells and during in vitro differentiation of human placental progenitor cells

PROVIDER: PRJNA476565 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7357230.fastq.gz	Fastqsanger.gz
	SRR7357231.fastq.gz	Fastqsanger.gz
	SRR7357232.fastq.gz	Fastqsanger.gz
	SRR7357233.fastq.gz	Fastqsanger.gz
	SRR7357234.fastq.gz	Fastqsanger.gz

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Sex-Specific Gene Expression Differences Are Evident in Human Embryonic Stem Cells and During In Vitro Differentiation of Human Placental Progenitor Cells.

Project description:The placenta is a short-lived tissue required for embryonic growth and survival, and it is fetal derived. Fetal sex influences gestation, and many sexual dimorphic diseases have origins in utero. There is sex-biased gene expression in third-trimester human placentas, yet the origin of sex-specific expression is unknown. Here, we used an in vitro differentiation model to convert human embryonic stem cells (hESCs) into trophoblastic progenitor cells of the first-trimester placenta, which will eventually become mature extravillous trophoblasts and syncytiotrophoblasts. We observed significant sex differences in transcriptomic profiles of hESCs and trophoblastic progenitors, and also with the differentiation process itself. Male cells had higher dosage of X/Y gene pairs relative to female samples, supporting functions for Y-linked genes beyond spermatogenesis in the hESCs and in the early placenta. Female-specific differentiation altered the expression of several thousand genes compared with male cells, and female cells specifically upregulated numerous autosomal genes with known roles in trophoblast function. Sex-biased upregulation of cellular pathways during trophoblast differentiation was also evident. This study is the first to identify sex differences in trophoblastic progenitor cells of the first-trimester human placenta, and reveal early origins for sexual dimorphism.

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Sex-specific gene expression differences are evident in human embryonic stem cells and during in vitro differentiation of human placental progenitor cells

Project description:Here, we examine sex-specific gene expression differences during in vitro differentiation of male and female human embryonic stem cells (hESCs) into trophoblastic progenitors, as our current understanding of the early events of human trophoblast formation is limited. We perform RNA-Sequencing in 3 female and 3 male hESC lines at days 0 and 5 of BMP4/A/P trophoblastic progenitor cell differentiation. Differential gene expression was performed with DESeq2, and reveals significant sex differences with transcriptomic profiles in hESCs and trophoblastic progenitors, and also with the differentiation process itself.

2018-12-31 | GSE115941 | GEO

Human Placental Transcriptome and fetal sex differences in Spontaneous Preterm Birth

Project description:Placental insufficiency is implicated in spontaneous preterm birth (SPTB). We performed RNA-seq study in male and female placentas from women (African American, self-identified) with SPTB (< 36 weeks gestation) compared to normal pregnancies (≥ 38 weeks gestation) to assess the alterations in gene expression profiles.

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Transcriptional signature of human placental mast cells

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Human Placental Transcriptome and fetal sex differences in Spontaneous Preterm Birth

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Ancestry and fetal sex differences in human placental transcriptome in spontaneous early preterm birth

Project description:Black women have highest risk of spontaneous preterm birth (SPTB) than other race, and placental insufficiency is implicated in SPTB. We performed RNA-seq study in male and female placentas from White and Black women (self-identified) with early SPTB (< 32 weeks gestation) compared to normal pregnancies (≥ 38 weeks gestation) to assess the alterations in gene expression profiles as well as ancestry and fetal sex differences. The mid-trimester placentas (19-23 weeks gestation) were used as gestational controls.

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Transcriptional signature of human placental mast cells

Project description:Uterine Mast cells (MCs) are essential for placentation, implantation and growth of fetus. In contrast, the functions of placental MCs are poorly investigated. The objective of the study was to evaluate the transcriptional profile of human placental MCs using whole genome microarray. We isolated MCs from placenta from healthy at term donors with CD117 and IgE antibodies, and used HMC-1.2 cell lines as controls. Data analysis revealed 16,468 genes modulated in human placenta MCs as compared with HMC-1.2 cell lines; most of them (83%) were up-regulated. The differentially expressed genes in placental MCs were enriched with three biological categories: immune response, FcReI signaling and reproduction processes. The two first categories revealed the importance of immune gene expression including chemokines, cytokines, interferon, and major histocompatibility complex antigens in placental MCs. A set of genes were modulated in FcReI process suggesting a role of IgE-mediated activation. Finally, in pregnancy category, we identified specific genes of WNT pathway and those involved in response to estrogen and progesterone. Taken together, human placental MCs exhibited a unique transcriptional profile enriched with immune response and pregnancy genes, which reflects the impact of placenta environment on MC functions.

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