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Single cell RNA sequencing of cultured CCR10+ and CCR10- lung airway epithelial cells

ABSTRACT: Single cell RNA sequencing of cultured CCR10+ and CCR10- lung airway epithelial cells

PROVIDER: PRJNA476693 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7444152.fastq.gz	Fastqsanger.gz
	SRR7444153.fastq.gz	Fastqsanger.gz

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Single cell RNA sequencing of cultured CCR10+ and CCR10- lung airway epithelial cells

Project description:Mechanically dissociated IPF lung explant derived cellular suspensions were cultured in 50% senescent lung fibroblast conditioned medium + 50% fibroblast complete medium (DMEM + 15% FBS + antiboitics) and 10 µM of Y27632. After approximately 2 weeks, cells were passaged and cultured overnight in Pneumacult Ex Plus medium (STEMCELL technologies).

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