Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional In vivo Recording and Single-cell Transcriptome Analysis Reveals Molecular Properties of Light-Sensitive Neurons in Mouse V1

ABSTRACT: Functional In vivo Recording and Single-cell Transcriptome Analysis Reveals Molecular Properties of Light-Sensitive Neurons in Mouse V1

PROVIDER: PRJNA476774 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7367040_1.fastq.gz	Fastqsanger.gz
	SRR7367040_2.fastq.gz	Fastqsanger.gz
	SRR7367050_1.fastq.gz	Fastqsanger.gz
	SRR7367050_2.fastq.gz	Fastqsanger.gz
	SRR7367060_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 126

Similar Datasets

Functional In vivo Recording and Single-cell Transcriptome Analysis Reveals Molecular Properties of Light-Sensitive Neurons in Mouse V1

Project description:We established a protocol of functional in vivo single-cell transcriptome (FIST) analysis

2020-05-03 | GSE115997 | GEO

Integrative analysis of in vivo recording with single-cell RNA-seq data reveals molecular properties of light-sensitive neurons in mouse V1.

Project description:Vision formation is classically based on projections from retinal ganglion cells (RGC) to the lateral geniculate nucleus (LGN) and the primary visual cortex (V1). Neurons in the mouse V1 are tuned to light stimuli. Although the cellular information of the retina and the LGN has been widely studied, the transcriptome profiles of single light-stimulated neuron in V1 remain unknown. In our study, in vivo calcium imaging and whole-cell electrophysiological patch-clamp recording were utilized to identify 53 individual cells from layer 2/3 of V1 as light-sensitive (LS) or non-light-sensitive (NS) by single-cell light-evoked calcium evaluation and action potential spiking. The contents of each cell after functional tests were aspirated in vivo through a patch-clamp pipette for mRNA sequencing. Moreover, the three-dimensional (3-D) morphological characterizations of the neurons were reconstructed in a live mouse after the whole-cell recordings. Our sequencing results indicated that V1 neurons with a high expression of genes related to transmission regulation, such as Rtn4r and Rgs7, and genes involved in membrane transport, such as Na+/K+ ATPase and NMDA-type glutamatergic receptors, preferentially responded to light stimulation. Furthermore, an antagonist that blocks Rtn4r signals could inactivate the neuronal responses to light stimulation in live mice. In conclusion, our findings of the vivo-seq analysis indicate the key role of the strength of synaptic transmission possesses neurons in V1 of light sensory.

| S-EPMC7251024 | biostudies-literature

V1 vs V1 FlhA knockout

Project description:Competitive hybridization of V1 variant of genome sequenced C. jejuni strain 11168 versus V1 variant in which flhA gene has been disrupted. Keywords: repeat sample

2004-03-03 | GSE530 | GEO

V1 vs V1 FliA knockout

Project description:Competive hybridization of V1 variant of genome sequenced C. jejuni strain 11168 versus V1 variant in which fliA gene encoding sigma 28 has been disrupted Keywords: repeat sample

2004-03-03 | GSE521 | GEO

Agrobacterium sp. V1 strain:V1

Project description:Agrobacterium sp. V1 strain:V1 Genome sequencing and assembly

| PRJNA984195 | ENA

Single-cell RNA sequencing of aspirates from cortical neurons after patch clamp recording

Project description:We obtained full transcriptome data from single cortical neurons after whole-cell patch-clamp recording (termed “Patch-seq”). By applying “Patch-seq” to cortical neurons, we reveal a close link between biophysical membrane properties and genes coding for neurotransmitter receptors and channels, including well-established and hitherto undescribed subtypes.

2015-12-21 | GSE70844 | GEO

V1 variant vs. V26 variant

Project description:Competitive hybridization of V1 variant versus V26 variant of genome sequenced C. jejuni strain 11168 Keywords: repeat sample

2004-03-03 | GSE531 | GEO

Expression data from V1 and dI4/dILA spinal interneurons

Project description:Spinal inhibitory interneurons play crucial roles in shaping motor output, but the molecular heterogeneity contained within cardinal spinal interneuron populations is unclear. This experiment was designed to identify genes enriched in the Engrailed1 (En1) population of ventral V1 inhibitory interneurons relative to the Ptf1a population of dorsal dI4/dILA interneurons, with the goal of identifying genetic markers for discrete V1 subpopulations.

2016-01-15 | GSE69560 | GEO

Nanostring nCounter human miRNA assay (v1) of eosinophilic esophagitis

Project description:Nanostring nCounter Human miRNA assay (v1) of esophageal mucosal biopsies from children with eosinophilic esophagitis and controls

2014-09-01 | GSE55794 | GEO

Hemerocallis middendorfii v1

Project description:Hemerocallis middendorfii v1 Genome sequencing and assembly

| PRJNA905889 | ENA