Project description:ABX464, a new drug for curing HIV and treating inflammatory diseases induces upregulation of the anti-inflammatory miR-124. We used microarrays to show the implication of ABX464 in the biogenesis of small noncoding RNAs. So, we decided to evaluate if miRNAs or small nucleolar RNAs (snoRNAs) were differentially regulated by ABX464.
Project description:RNA-sequencing was performed on baseline blood samples from HIV-infected and HIV-uninfected asymptomatic adults with recent household exposure to an index case of infectious pulmonary tuberculosis (TB) and with detectable Mtb DNA in PBMC. Additional sequencing was also performed on follow-up blood samples from HIV-infected participants following completion of isoniazid preventative therapy.
Project description:Studying microRNAs in the immune cells of athletes offers a novel perspective on the molecular regulation of immune function and recovery, potentially uncovering strategies to enhance performance and resilience to physical stress. However, PBMC microRNA expression in endurance athletes, such as runners and cyclists, remains underexplored, especially with regard to sex differences. This study aimed to (i) assess sport- and sex-specific differences in PBMC microRNA expression induced by acute aerobic exercise in runners and cyclists, (ii) evaluate the impact of maximal (trial A) and sub-maximal (trial B) exercises, and (iii) examine correlations between PBMC microRNAs and exercise performance. Methods: A total of 58 participants were included: 22 runners (9 females), 18 cyclists (9 females), and 18 active controls (9 females). Participants underwent VO2max and time-to-exhaustion tests, with blood samples collected pre- and post-exercise to analyze PBMC microRNA levels. Results: Runners exhibited a stronger microRNA response than cyclists or controls, with significant sex-based differences. After maximal intensity exercise, 279 microRNAs (255 upregulated) were altered in runners, compared to only 7 microRNAs (none upregulated) in cyclists. Exercise intensity and duration had sport-specific effects on microRNA expression. Time-to-exhaustion in runners and weekly training volume in both groups were significantly associated with changes in PBMC microRNA profiles. Conclusion: This study reveals that PBMC microRNA expression in response to acute aerobic exercise is sport- and sex-specific, providing new insights into the molecular adaptations of endurance athletes and their relationship to athletic performance.
Project description:The expression of 30362 plant genes from uninfected flowers of Boechera stricta, uninfected steam and leaves of B. stricta and infected B. stricta with Puccinia monoica forming pseudoflowers. We hybridized cDNA from each sample to an Arabidopsis thaliana gene expression 4x72K format NimbleGen array (ATH6_60mer_expr).
Project description:Frozen PBMC from infants 7 days before randomization (PBMC from two infant were 28 days after randomization) were stimulated in RPMI with 10% Feotal calf serum (FCS) at 5 million/ml with 1 million CFU/ml BCG (SII) or left unstimulated in a total volumn of 200ul/well in 96-well plates. Cells were incubated for 12 hours (37C, 5% CO2). Plates were centrifuged and 200ul supernatant was removed without disrupting the pellet and transferred into clean U-bottom plates. The plates were centrifuged again, the supernatant were flicked-out and the cells were resuspended in 200ul RLT buffer with beta-mercaptoethanol (10ul/ml). The samples were mixed with the buffer to lyse the cells and the plates were sealed and stored at -20C. At the time of RNA-extraction, plates were thawed at 37% for 10 mins. RNA extraction was performed using the Qiagen RNeasy kit following manufacturer's instructions (including optional DNase digestion) and RNA was eluted twice, 30 ul per elution. RNA was quantified by nanodrop and samples were stored at -80C.
Project description:This study aimed to identify potential protein biomarkers for antemortem diagnosis of rabies in dogs, which are the principal reservoir hosts of the rabies virus. Lyssavirus rabies, the prototype species, effectively evades the host immune response allowing the infection to progress unnoticed until the onset of clinical signs. At this stage, the disease is irreversible and invariably fatal, with definitive diagnosis possible only post-mortem. Two hundred and thirty-one samples of brain tissue, cerebrospinal fluid, and serum were collected from apparently healthy dogs brought for slaughter for human consumption in South-East and North-Central Nigeria. All the brain tissue were subjected to a direct fluorescent antibody test to confirm the presence of lyssavirus antigen, and 8.7% (n = 20) were positive. Protein extraction and quantification was performed on 20 rabies-infected and -uninfected samples from each sample type, and only 10 rabies-infected and -uninfected samples were selected from each sample type as they had sufficient protein quantity for further sample preparation. Reduction and alkylation of the selected samples was performed and on-bead HILIC sample clean-up and tryptic digestion followed thereafter. The resulting peptides from each sample were injected into the EvoSep One LC system, coupled to the timsTOF HT mass spectrometer, using the standard dia-PASEF short gradient data acquisition method. Data was processed using Spectronaut (v19) and an unpaired t-test was performed to compare identified protein groups (proteins and their isoforms) between the rabies-infected and uninfected brain tissue, cerebrospinal fluid, and serum samples.