Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Specific Parallel Analysis of RNA End and Small RNA libraries

ABSTRACT: Specific Parallel Analysis of RNA End and Small RNA libraries

PROVIDER: PRJNA478317 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6930533.fastq.gz	Fastqsanger.gz
	SRR6930534.fastq.gz	Fastqsanger.gz
	SRR6930535.fastq.gz	Fastqsanger.gz
	SRR6930536.fastq.gz	Fastqsanger.gz
	SRR6930538.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Small RNA libraries

Project description:Small RNA libraries

| PRJNA478199 | ENA

Specific Parallel Analysis of RNA End and Small RNA libraries

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-09-11 | GSE116376 | GEO

Massively parallel splicing reporter assay on designed sequence libraries

Project description:Massively parallel splicing reporter assay on designed sequence libraries

| PRJNA545827 | ENA

Improved characterization of single-cell RNA-seq libraries with paired-end avidity sequencing

Project description:Improved characterization of single-cell RNA-seq libraries with paired-end avidity sequencing

| PRJNA1140622 | ENA

Specific Parallel Analysis of RNA Ends

Project description:Specific Parallel Analysis of RNA Ends

| PRJNA448580 | ENA

Massively parallel quantification of SpCas9 gRNA efficiencies by surrogate lentiviral libraries

Project description:Massively parallel quantification of SpCas9 gRNA efficiencies by surrogate lentiviral libraries

| PRJNA726810 | ENA

Massively parallel reporter assay for programmed ribosomal frameshifting on designed sequence libraries

Project description:Massively parallel reporter assay for programmed ribosomal frameshifting on designed sequence libraries

| PRJNA607955 | ENA

Zebrafish Paired End DiTag libraries

Project description:Used a DNA tag sequencing and mapping strategy called gene identification signature (GIS) analysis, in which 5' and 3' signatures of full-length cDNAs are accurately extracted into paired-end ditags (PETs) that are concatenated for efficient sequencing and mapped to genome sequences to demarcate the transcription boundaries of every gene. GIS analysis is potentially 30-fold more efficient than standard cDNA sequencing approaches for transcriptome characterization. Keywords: Paired End DiTags

2008-12-01 | GSE6694 | GEO

Multiple insert size paired-end sequencing for deconvolution of complex transcriptomes

Project description:Deep sequencing of transcriptomes allows quantitative and qualitative analysis of many RNA species in a sample, with parallel comparison of expression levels, splicing variants, natural antisense transcripts, RNA editing and transcriptional start and stop sites the ideal goal. By computational modeling, we show how libraries of multiple insert sizes combined with strand-specific, paired-end (SS-PE) sequencing can increase the information gained on alternative splicing, especially in higher eukaryotes. Despite the benefits of gaining SS-PE data with paired ends of varying distance, the standard Illumina protocol allows only non-strand-specific, paired-end sequencing with a single insert size. Here, we modify the Illumina RNA ligation protocol to allow SS-PE sequencing by using a custom pre-adenylated 3’ adaptor. We generate parallel libraries with differing insert sizes to aid deconvolution of alternative splicing events and to characterize the extent and distribution of natural antisense transcription in C. elegans. Despite stringent requirements for detection of alternative splicing, our data increases the number of intron retention and exon skipping events annotated in the Wormbase genome annotations by 127 % and 121 %, respectively. We show that parallel libraries with a range of insert sizes increase transcriptomic information gained by sequencing and that by current established benchmarks our protocol gives competitive results with respect to library quality.

2012-08-31 | GSE40507 | GEO

Transposon-based construction of strand-specific RNA-seq libraries

Project description:We have developed two methods for efficiently consructing RNA-seq libraries using transposition. Each method constructs high quality RNA-seq libraries when compared to standard approaches. One of the methods (Directional Tn-RNA-seq) maintains strand-of-origin information and exhibits strand specificity comparable to current approaches.

2011-10-10 | GSE32307 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data